Consequences of the deletion of the major specialized metabolite biosynthetic pathways of Streptomyces coelicolor on the metabolome and lipidome of this strain.
Journal
Microbial biotechnology
ISSN: 1751-7915
Titre abrégé: Microb Biotechnol
Pays: United States
ID NLM: 101316335
Informations de publication
Date de publication:
Aug 2024
Aug 2024
Historique:
received:
26
01
2024
accepted:
13
07
2024
medline:
2
8
2024
pubmed:
2
8
2024
entrez:
2
8
2024
Statut:
ppublish
Résumé
Chassis strains, derived from Streptomyces coelicolor M145, deleted for one or more of its four main specialized metabolites biosynthetic pathways (CPK, CDA, RED and ACT), in various combinations, were constructed for the heterologous expression of specialized metabolites biosynthetic pathways of various types and origins. To determine consequences of these deletions on the metabolism of the deleted strains comparative lipidomic and metabolomic analyses of these strains and of the original strain were carried out. These studies unexpectedly revealed that the deletion of the peptidic clusters, RED and/or CDA, in a strain deleted for the ACT cluster, resulted into a great increase in the triacylglycerol (TAG) content, whereas the deletion of polyketide clusters, ACT and CPK had no impact on TAG content. Low or high TAG content of the deleted strains was correlated with abundance or paucity in amino acids, respectively, reflecting high or low activity of oxidative metabolism. Hypotheses based on what is known on the bio-activity and the nature of the precursors of these specialized metabolites are proposed to explain the unexpected consequences of the deletion of these pathways on the metabolism of the bacteria and on the efficiency of the deleted strains as chassis strains.
Identifiants
pubmed: 39093579
doi: 10.1111/1751-7915.14538
doi:
Substances chimiques
Triglycerides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14538Subventions
Organisme : PROBIO3 "Investment for the Future Biotechnologies et Bioressources"
ID : n°11-BTBR-0003
Organisme : ANR INNOVANTIBIO from the "Direction Générale des Armées" (DGA) et "l'Agence de l'Innovation de Défense" (AID)
ID : ANR-17-ASTR-0018
Organisme : ANR BioSound-IR
ID : ANR-15-CE09-0002
Organisme : Public Institutions: CNRS and University Paris-Saclay
Informations de copyright
© 2024 The Author(s). Microbial Biotechnology published by John Wiley & Sons Ltd.
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