Underlying mechanism of Dendrobium huoshanense resistance to lead stress using the quantitative proteomics method.
Anabolism
Antioxidant
Free radical burst
Heavy metal
Stress tolerance
Journal
BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807
Informations de publication
Date de publication:
06 Aug 2024
06 Aug 2024
Historique:
received:
10
05
2024
accepted:
01
08
2024
medline:
6
8
2024
pubmed:
6
8
2024
entrez:
5
8
2024
Statut:
epublish
Résumé
Lead affects photosynthesis and growth and has serious toxic effects on plants. Here, the differential expressed proteins (DEPs) in D. huoshanense were investigated under different applications of lead acetate solutions. Using label-free quantitative proteomics methods, more than 12,000 peptides and 2,449 proteins were identified. GO and KEGG functional annotations show that these differential proteins mainly participate in carbohydrate metabolism, energy metabolism, amino acid metabolism, translation, protein folding, sorting, and degradation, as well as oxidation and reduction processes. A total of 636 DEPs were identified, and lead could induce the expression of most proteins. KEGG enrichment analysis suggested that proteins involved in processes such as homologous recombination, vitamin B6 metabolism, flavonoid biosynthesis, cellular component organisation or biogenesis, and biological regulation were significantly enriched. Nearly 40 proteins are involved in DNA replication and repair, RNA synthesis, transport, and splicing. The effect of lead stress on D. huoshanense may be achieved through photosynthesis, oxidative phosphorylation, and the production of excess antioxidant substances. The expression of 9 photosynthesis-related proteins and 12 oxidative phosphorylation-related proteins was up-regulated after lead stress. Furthermore, a total of 3 SOD, 12 POD, 3 CAT, and 7 ascorbate-related metabolic enzymes were identified. Under lead stress, almost all key enzymes involved in the synthesis of antioxidant substances are up-regulated, which may facilitate the scavenging of oxygen-free radical scavenging. The expression levels of some key enzymes involved in sugar and glycoside synthesis, the phenylpropanoid synthesis pathway, and the terpene synthesis pathway also increased. More than 30 proteins involved in heavy metal transport were also identified. Expression profiling revealed a significant rise in the expression of the ABC-type multidrug resistance transporter, copper chaperone, and P-type ATPase with exposure to lead stress. Our findings lay the basis for research on the response and resistance of D. huoshanense to heavy metal stress.
Identifiants
pubmed: 39103795
doi: 10.1186/s12870-024-05476-9
pii: 10.1186/s12870-024-05476-9
doi:
Substances chimiques
Plant Proteins
0
Lead
2P299V784P
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
748Subventions
Organisme : Open Fund of Anhui Engineering Research Center for Eco-agriculture of Traditional Chinese Medicine
ID : WXZR202318
Organisme : Demonstration Experiment Training Center of Anhui Provincial Department of Education
ID : 2022sysx033
Organisme : Startup fund for high-level talents of West Anhui University
ID : WGKQ2022025
Organisme : National Health Commission Scientific Research Fund
ID : SBGJ202301010
Informations de copyright
© 2024. The Author(s).
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