Cyclin F-EXO1 axis controls cell cycle-dependent execution of double-strand break repair.

DNA Breaks, Double-Stranded Humans Cell Cycle / genetics DNA Repair Exodeoxyribonucleases / metabolism Cyclins / metabolism DNA Repair Enzymes / metabolism DNA End-Joining Repair Ubiquitination Radiation, Ionizing

Journal

Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440

Informations de publication

Date de publication:
09 Aug 2024
Historique:
medline: 9 8 2024
pubmed: 9 8 2024
entrez: 9 8 2024
Statut: ppublish

Résumé

Ubiquitination is a crucial posttranslational modification required for the proper repair of DNA double-strand breaks (DSBs) induced by ionizing radiation (IR). DSBs are mainly repaired through homologous recombination (HR) when template DNA is present and nonhomologous end joining (NHEJ) in its absence. In addition, microhomology-mediated end joining (MMEJ) and single-strand annealing (SSA) provide backup DSBs repair pathways. However, the mechanisms controlling their use remain poorly understood. By using a high-resolution CRISPR screen of the ubiquitin system after IR, we systematically uncover genes required for cell survival and elucidate a critical role of the E3 ubiquitin ligase SCF

Identifiants

DOI: 10.1126/sciadv.ado0636 PMID: 39121215
pubmed: 39121215
doi: 10.1126/sciadv.ado0636
doi:

Substances chimiques

Exodeoxyribonucleases EC 3.1.-
Cyclins 0
EXO1 protein, human EC 3.1.-
DNA Repair Enzymes EC 6.5.1.-
CCNF protein, human 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

eado0636

Auteurs

Hongbin Yang (H)

MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK.
ORCID: 0000-0001-7757-9178

Shahd Fouad (S)

MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK.

Paul Smith (P)

MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK.
Edinburgh Cancer Research, CRUK Scotland Centre, Institute of Genetics and Cancer, University of Edinburgh, Crewe Road South, Edinburgh EH4 2XU, UK.
ORCID: 0000-0003-1079-9330

Eun Young Bae (EY)

MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK.
ORCID: 0009-0002-7294-0141

Yu Ji (Y)

MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK.
ORCID: 0009-0004-6257-180X

Xinhui Lan (X)

MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK.
Edinburgh Cancer Research, CRUK Scotland Centre, Institute of Genetics and Cancer, University of Edinburgh, Crewe Road South, Edinburgh EH4 2XU, UK.
ORCID: 0009-0005-1618-4881

Ava Van Ess (A)

MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK.
Edinburgh Cancer Research, CRUK Scotland Centre, Institute of Genetics and Cancer, University of Edinburgh, Crewe Road South, Edinburgh EH4 2XU, UK.
ORCID: 0000-0003-2473-5717

Francesca M Buffa (FM)

MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK.
Medical Science Division, University of Oxford, Oxford OX3 7DQ, UK.
Department of Computing Sciences and the Bocconi Institute for Data Science and Analytics, Bocconi University, Milan, Italy.
ORCID: 0000-0003-0409-406X

Roman Fischer (R)

Target Discovery Institute, Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Oxford OX3 7FZ, UK.
Chinese Academy for Medical Sciences Oxford Institute, Nuffield Department of Medicine, University of Oxford, Roosevelt Drive, Oxford OX3 7FZ, UK.
ORCID: 0000-0002-9715-5951

Iolanda Vendrell (I)

Target Discovery Institute, Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Oxford OX3 7FZ, UK.
Chinese Academy for Medical Sciences Oxford Institute, Nuffield Department of Medicine, University of Oxford, Roosevelt Drive, Oxford OX3 7FZ, UK.
ORCID: 0000-0001-7187-4061

Benedikt M Kessler (BM)

Target Discovery Institute, Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Oxford OX3 7FZ, UK.
Chinese Academy for Medical Sciences Oxford Institute, Nuffield Department of Medicine, University of Oxford, Roosevelt Drive, Oxford OX3 7FZ, UK.
ORCID: 0000-0002-8160-2446

Vincenzo D'Angiolella (V)

MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK.
Edinburgh Cancer Research, CRUK Scotland Centre, Institute of Genetics and Cancer, University of Edinburgh, Crewe Road South, Edinburgh EH4 2XU, UK.
ORCID: 0000-0001-8365-9094

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Altération de l'ADN › Cassures de l'ADN › Cassures double-brin de l'ADN › Cyclin F-EXO1 axis controls cell...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Cyclin F-EXO1 axis controls cell...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Cycle cellulaire › Cyclin F-EXO1 axis controls cell...
questionsmedicales.fr › Phénomènes génétiques › Réparation de l'ADN › Cyclin F-EXO1 axis controls cell...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Exonucleases › Exodeoxyribonucleases › Cyclin F-EXO1 axis controls cell...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Cyclines › Cyclin F-EXO1 axis controls cell...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Enzymes de réparation de l'ADN › Cyclin F-EXO1 axis controls cell...
questionsmedicales.fr › Phénomènes génétiques › Réparation de l'ADN › Réparation de l'ADN par jonction d'extrémités › Cyclin F-EXO1 axis controls cell...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Modification traductionnelle des protéines › Maturation post-traductionnelle des protéines › Ubiquitination › Cyclin F-EXO1 axis controls cell...
questionsmedicales.fr › Phénomènes physiques › Rayonnement › Rayonnement ionisant › Cyclin F-EXO1 axis controls cell...