An in vitro assay to explore condensation domain specificity from non-ribosomal peptide synthesis.

Substrate Specificity Siderophores / chemistry Peptide Synthases / metabolism Peptides / chemistry Peptide Biosynthesis, Nucleic Acid-Independent Catalytic Domain
Condensation domain Fuscachelin NRPS Non-ribosomal peptide synthesis SpyTag/SpyCatcher

Journal

Methods in enzymology
ISSN: 1557-7988
Titre abrégé: Methods Enzymol
Pays: United States
ID NLM: 0212271

Informations de publication

Date de publication:
2024
Historique:
medline: 19 8 2024
pubmed: 19 8 2024
entrez: 18 8 2024
Statut: ppublish

Résumé

Non-ribosomal peptide synthesis produces a wide range of bioactive peptide natural products and is reliant on a modular architecture based on repeating catalytic domains able to generate diverse peptide sequences. In this chapter we detail an in vitro biochemical assay to explore the substrate specificity of condensation domains, which are responsible for peptide elongation, from the biosynthetic machinery that produces from the siderophore fuscachelin. This assay removes the requirement to utilise the specificity of adjacent adenylation domains and allows the acceptance of a wide range of synthetic substrates to be explored.

Identifiants

DOI: 10.1016/bs.mie.2024.06.010 PMID: 39155122
pubmed: 39155122
pii: S0076-6879(24)00303-3
doi: 10.1016/bs.mie.2024.06.010
pii:
doi:

Substances chimiques

Siderophores 0
Peptide Synthases EC 6.3.2.-
Peptides 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

89-119

Informations de copyright

Copyright © 2024. Published by Elsevier Inc.

Auteurs

Minuri Ratnayake (M)

Department of Biochemistry and Molecular Biology, The Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia; EMBL Australia, Monash University, Clayton, VIC, Australia; ARC Centre of Excellence for Innovations in Peptide and Protein Science.

Y T Candace Ho (YTC)

Department of Chemistry, University of Warwick, Coventry, United Kingdom.

Xinyun Jian (X)

Department of Biochemistry and Molecular Biology, The Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia; EMBL Australia, Monash University, Clayton, VIC, Australia; ARC Centre of Excellence for Innovations in Peptide and Protein Science.

Max J Cryle (MJ)

Department of Biochemistry and Molecular Biology, The Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia; EMBL Australia, Monash University, Clayton, VIC, Australia; ARC Centre of Excellence for Innovations in Peptide and Protein Science. Electronic address: max.cryle@monash.edu.

Articles similaires

Proteomimetic polymer blocks mitochondrial damage, rescues Huntington's neurons, and slows onset of neuropathology in vivo.

Wonmin Choi, Mara Fattah, Yutong Shang et al.
1.00
Animals Huntington Disease Mitochondria Neurons Mice

Mutational analysis of Phanerochaete chrysosporium´s purine transporter.

Mariana Barraco-Vega, Manuel Sanguinetti, Gabriela da Rosa et al.
1.00
Phanerochaete Fungal Proteins Purines Aspergillus nidulans DNA Mutational Analysis

Evaluation of herbicidal potential of Siderophores produced by Amycolatopsis lurida strain 407.

Mahsa Eigharlou, Sara Javidpoor, Ali Mohammadi et al.
1.00
Siderophores Herbicides Plant Weeds Lolium Actinobacteria

PRR adjuvants restrain high stability peptides presentation on APCs.

Bin Li, Jin Zhang, Taojun He et al.
1.00
Animals Adjuvants, Immunologic Mice Antigen-Presenting Cells Antigen Presentation

Classifications MeSH

questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Spécificité du substrat › An in vitro assay to explore condensation...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Agents séquestrants › Chélateurs › Agents chélateurs du fer › Sidérophores › An in vitro assay to explore condensation...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Ligases › Carbon-nitrogen ligases › Amino-acid ligases › An in vitro assay to explore condensation...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › An in vitro assay to explore condensation...
questionsmedicales.fr › Métabolisme › Biosynthèse des peptides › Biosynthèse non ribosomique des peptides › An in vitro assay to explore condensation...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Éléments structuraux des protéines › Domaine catalytique › An in vitro assay to explore condensation...