The role of DNA polymerase I in tolerating single-strand breaks generated at clustered DNA damage in Escherichia coli.
Clustered DNA damage
DNA polymerase I
Single-strand break
Template-strand preference
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
18 Aug 2024
18 Aug 2024
Historique:
received:
17
05
2024
accepted:
08
08
2024
medline:
19
8
2024
pubmed:
19
8
2024
entrez:
18
8
2024
Statut:
epublish
Résumé
Clustered DNA damage, when multiple lesions are generated in close proximity, has various biological consequences, including cell death, chromosome aberrations, and mutations. It is generally perceived as a hallmark of ionizing radiation. The enhanced mutagenic potential of lesions within a cluster has been suggested to result, at least in part, from the selection of the strand with the mutagenic lesion as the preferred template strand, and that this process is relevant to the tolerance of persistent single-strand breaks generated during an attempted repair. Using a plasmid-based assay in Escherichia coli, we examined how the strand bias is affected in mutant strains deficient in different DNA polymerase I activities. Our study revealed that the strand-displacement and 5'-flap endonuclease activities are required for this process, while 3'-to-5' exonuclease activity is not. We also found the strand template that the mutagenic lesion was located on, whether lagging or leading, had no effect on this strand bias. Our results imply that an unknown pathway operates to repair/tolerate the single-strand break generated at a bi-stranded clustered damage site, and that there exist different backup pathways, depending on which DNA polymerase I activity is compromised.
Identifiants
pubmed: 39155334
doi: 10.1038/s41598-024-69823-4
pii: 10.1038/s41598-024-69823-4
doi:
Substances chimiques
DNA Polymerase I
EC 2.7.7.7
Escherichia coli Proteins
0
DNA, Bacterial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
19124Subventions
Organisme : KAKENHI
ID : 17K20052
Organisme : KAKENHI
ID : 21K18148
Informations de copyright
© 2024. The Author(s).
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