Fusiform nanoparticle boosts efficient genetic transformation in Sclerotinia sclerotiorum.
Sclerotinia sclerotiorum
Ss-oah1
Fusiform nanoparticle
Gene silencing
Mycelium transformation
Journal
Journal of nanobiotechnology
ISSN: 1477-3155
Titre abrégé: J Nanobiotechnology
Pays: England
ID NLM: 101152208
Informations de publication
Date de publication:
20 Aug 2024
20 Aug 2024
Historique:
received:
17
05
2024
accepted:
23
07
2024
medline:
20
8
2024
pubmed:
20
8
2024
entrez:
19
8
2024
Statut:
epublish
Résumé
Sclerotinia sclerotiorum is a highly destructive phytopathogenic fungus that poses a significant threat to a wide array of crops. The current constraints in genetic manipulation techniques impede a thorough comprehension of its pathogenic mechanisms and the development of effective control strategies. Herein, we present a highly efficient genetic transformation system for S. sclerotiorum, leveraging the use of fusiform nanoparticles, which are synthesized with FeCl Our findings demonstrate the feasibility of using nanoparticle-mediated delivery as a rapid and reliable tool for genetic modification in S. sclerotiorum. Given its simplicity and high efficiency, it has the potential to significantly propel genetic research in filamentous fungi, offering new avenues for elucidating the intricacies of pathogenicity and developing innovative disease management strategies.
Sections du résumé
BACKGROUND
BACKGROUND
Sclerotinia sclerotiorum is a highly destructive phytopathogenic fungus that poses a significant threat to a wide array of crops. The current constraints in genetic manipulation techniques impede a thorough comprehension of its pathogenic mechanisms and the development of effective control strategies.
RESULTS
RESULTS
Herein, we present a highly efficient genetic transformation system for S. sclerotiorum, leveraging the use of fusiform nanoparticles, which are synthesized with FeCl
CONCLUSIONS
CONCLUSIONS
Our findings demonstrate the feasibility of using nanoparticle-mediated delivery as a rapid and reliable tool for genetic modification in S. sclerotiorum. Given its simplicity and high efficiency, it has the potential to significantly propel genetic research in filamentous fungi, offering new avenues for elucidating the intricacies of pathogenicity and developing innovative disease management strategies.
Identifiants
pubmed: 39160572
doi: 10.1186/s12951-024-02736-6
pii: 10.1186/s12951-024-02736-6
doi:
Substances chimiques
Pyrimidines
0
Fungal Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
494Subventions
Organisme : Natural Science Foundation of Chongqing
ID : CSTB2023NSCQ-MSX0355
Organisme : the National Key Research and Development Progragm of China
ID : 2023YFF1000700
Organisme : the Fundamental Research Funds for the Central Universities
ID : SWU120075
Organisme : National Natural Science Foundation of China
ID : 32372077
Informations de copyright
© 2024. The Author(s).
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