Association of pathogenic determinants of Fusobacterium necrophorum with bacteremia, and Lemierre's syndrome.
Fusobacterium necrophorum
/ genetics
Humans
Lemierre Syndrome
/ microbiology
Bacteremia
/ microbiology
Virulence Factors
/ genetics
Male
Female
Phylogeny
Adult
Genome-Wide Association Study
Middle Aged
Bacterial Proteins
/ genetics
Fusobacterium Infections
/ microbiology
Aged
Genomic Islands
/ genetics
Hemolysin Proteins
Anaerobes
Bacteremia
Fusobacterium
Lemierre’s syndrome
Sepsis
Thrombophlebitis
Tonsillitis
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
27 Aug 2024
27 Aug 2024
Historique:
received:
22
04
2024
accepted:
19
08
2024
medline:
28
8
2024
pubmed:
28
8
2024
entrez:
27
8
2024
Statut:
epublish
Résumé
Fusobacterium necrophorum is a Gram-negative anaerobic bacterium responsible for localized infections of the oropharynx that can evolve into bacteremia and/or septic thrombophlebitis of the jugular vein or peritonsillar vein, called Lemierre's syndrome. To identify microbial genetic determinants associated with the severity of this life-threatening disease, 70 F. necrophorum strains were collected and grouped into two categories according to the clinical presentation: (i) localized infection, (ii) bacteremia with/without Lemierre's syndrome. Comparative genomic analyses revealed two clades with distinct genetic content, one clade being significantly enriched with isolates from subjects with bacteremia. To identify genetic determinants contributing to F. necrophorum pathogenicity, genomic islands and virulence factor orthogroups (OVFs) were predicted. The presence/absence profiles of OVFs did not group isolates according to their clinical category, but rather according to their phylogeny. However, a variant of lktA, a key virulence factor, with a frameshift deletion that results in two open reading frames, was associated with bacteremia. Moreover, a genome-wide association study identified three orthogroups associated with bacteremic strains: (i) cas8a1, (ii) a sodium/solute symporter, and (iii) a POP1 domain-containing protein. Further studies must be performed to assess the functional impact of lktA mutation and of these orthogroups on the physiopathological mechanisms of F. necrophorum infections.
Identifiants
pubmed: 39191804
doi: 10.1038/s41598-024-70608-y
pii: 10.1038/s41598-024-70608-y
doi:
Substances chimiques
Virulence Factors
0
Bacterial Proteins
0
lktA protein, bacteria
0
Hemolysin Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
19804Informations de copyright
© 2024. The Author(s).
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