First insight into the whole genome sequence variations in clarithromycin resistant Helicobacter pylori clinical isolates in Russia.
Clarithromycin
/ pharmacology
Helicobacter pylori
/ genetics
Russia
/ epidemiology
Humans
Drug Resistance, Bacterial
/ genetics
Anti-Bacterial Agents
/ pharmacology
Microbial Sensitivity Tests
Whole Genome Sequencing
/ methods
Helicobacter Infections
/ microbiology
Mutation
RNA, Ribosomal, 23S
/ genetics
Genome, Bacterial
Male
Genetic Variation
Female
Adult
Middle Aged
Aged
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
29 Aug 2024
29 Aug 2024
Historique:
received:
26
01
2024
accepted:
22
08
2024
medline:
31
8
2024
pubmed:
31
8
2024
entrez:
29
8
2024
Statut:
epublish
Résumé
Clarithromycin (CLR) is currently a key antibiotic for Helicobacter pylori infection treatment, however, the data on CLR resistance patterns in Russia are missing. Here, we applied WGS-based approach to H. pylori clinical isolates from Russia to comprehensively investigate sequence variation, identify putative markers of CLR resistance and correlate them with phenotypic susceptibility testing. The phenotypic susceptibility of 44 H. pylori isolates (2014-2022) to CLR was determined by disc diffusion method: 23 isolates were CLR-resistant and 21-CLR-susceptible. All isolates were subjected to WGS and submitted to GenBank. Based on complete sequence analysis, we showed that among all sequence variants, the combination of mutations A2146G/A2147G in the 23S rRNA gene is the most reliable for prediction of phenotypic susceptibility. For the first time, the average number of mutations in 106 virulence-associated genes between resistant and susceptible groups were compared. Moreover, this study presents the first WGS insight into genetic diversity of H. pylori in Russia with a particular focus on the molecular basis of drug resistance: the novel mutations were described as potential markers for the resistance development. Of these, the most prominent was a frameshift deletion (252:CGGGT) in HP0820 coding region, which is a good candidate for further investigation.
Identifiants
pubmed: 39209935
doi: 10.1038/s41598-024-70977-4
pii: 10.1038/s41598-024-70977-4
doi:
Substances chimiques
Clarithromycin
H1250JIK0A
Anti-Bacterial Agents
0
RNA, Ribosomal, 23S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
20108Informations de copyright
© 2024. The Author(s).
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