Effects of 630 nm laser on apoptosis, metastasis, invasion and epithelial-to-mesenchymal transition of human lung squamous cell carcinoma H520 cells mediated by hematoporphyrin derivatives.
Humans
Epithelial-Mesenchymal Transition
/ drug effects
Apoptosis
/ drug effects
Lung Neoplasms
/ pathology
Cell Line, Tumor
Photochemotherapy
/ methods
Carcinoma, Squamous Cell
/ pathology
Cell Movement
/ drug effects
Cell Proliferation
/ drug effects
Neoplasm Invasiveness
Photosensitizing Agents
/ pharmacology
Hematoporphyrin Derivative
/ pharmacology
Proto-Oncogene Proteins c-bcl-2
/ metabolism
Cadherins
/ metabolism
Vimentin
/ metabolism
Caspase 9
/ metabolism
Apoptosis
Epithelial-mesenchymal transition
Hematoporphyrin derivatives
Human lung squamous carcinoma H520 cells
Metastasis
Photodynamic therapy
Journal
Lasers in medical science
ISSN: 1435-604X
Titre abrégé: Lasers Med Sci
Pays: England
ID NLM: 8611515
Informations de publication
Date de publication:
30 Aug 2024
30 Aug 2024
Historique:
received:
04
07
2024
accepted:
20
08
2024
medline:
31
8
2024
pubmed:
31
8
2024
entrez:
29
8
2024
Statut:
epublish
Résumé
Photodynamic therapy (PDT) has significant advantages in the treatment of malignant lung tumors. The research on the mechanism of PDT mediated by hematoporphyrin derivatives (HPD) and its cytotoxic effects on lung cancer cells has primarily focused on lung adenocarcinoma cells. However, the impact of HPD-PDT on lung squamous cell carcinoma has not been thoroughly studied. This study aimed to investigate the effects of 630 nm laser on apoptosis, metastasis, invasion, and epithelial-mesenchymal transition (EMT) in human lung squamous cell carcinoma H520 cells mediated by HPD. H520 cells were divided into four groups: control group, photosensitizer group, irradiation group, and HPD-PDT group. Cell proliferation was assessed using CCK8 assay; cell apoptosis was detected by Hoechst 33258 staining and flow cytometry; cell migration and invasion abilities were evaluated using wound-healing and invasion assays; and protein and mRNA expressions were analyzed by Western blot and reverse transcription-polymerase chain reaction (RT-PCR) respectively. Results showed that HPD-PDT significantly inhibited cell proliferation, promoted apoptosis (P < 0.05), suppressed cell migration and invasion (P < 0.05), decreased Bcl-2 mRNA expression, and increased Bax and Caspase-9 mRNA expression(P < 0.05). Western blotting analysis indicated increased expression of Bax, Caspase-9, and E-cadherin, and decreased expression of Bcl-2, N-cadherin, and Vimentin (P < 0.05). In conclusion, 630 nm laser mediated by HPD promoted cell apoptosis via upregulation of Bax and caspase-9, and downregulation of Bcl-2, and inhibited cell migration and invasion by regulating EMT in H520 cells.
Identifiants
pubmed: 39210165
doi: 10.1007/s10103-024-04176-y
pii: 10.1007/s10103-024-04176-y
doi:
Substances chimiques
Photosensitizing Agents
0
Hematoporphyrin Derivative
68335-15-9
Proto-Oncogene Proteins c-bcl-2
0
Cadherins
0
Vimentin
0
Caspase 9
EC 3.4.22.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
228Subventions
Organisme : Beijing Science and Technology Medical Development Foundation
ID : No. KC2021-JX-0186-64
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.
Références
Bray F et al (2024) Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: a Cancer Journal for clinicians. 74(3):229–263
Duma N, Santana-Davila R, Molina JR (2019) Non–small cell Lung Cancer: Epidemiology, Screening, diagnosis, and treatment. Mayo Clin Proc 94(8):1623–1640
doi: 10.1016/j.mayocp.2019.01.013
pubmed: 31378236
Nooreldeen R, Bach H (2021) Current and Future Development in Lung Cancer diagnosis. Int J Mol Sci, 22(16)
Niu Z et al (2022) Signaling pathways and targeted therapies in lung squamous cell carcinoma: mechanisms and clinical trials. Signal Transduct Target Therapy, 7(1)
Lau SCM et al (2022) Squamous cell lung cancer: current landscape and future therapeutic options. Cancer Cell 40(11):1279–1293
doi: 10.1016/j.ccell.2022.09.018
pubmed: 36270277
Wan Y et al (2021) Conquering the Hypoxia limitation for photodynamic therapy. Adv Mater, 33(48)
Correia JH et al (2021) Photodynamic therapy review: principles, photosensitizers, applications, and future directions. Pharmaceutics, 13(9)
Banerjee SM et al (2020) Photodynamic therapy in primary breast Cancer. J Clin Med, 9(2)
Juarranz Á, Gilaberte Y, González S (2020) Photodynamic therapy (PDT) in Oncology. Cancers, 12(11)
Shanazarov NA et al (2024) Photodynamic therapy of cervical cancer: a scoping review on the efficacy of various molecules. Therapeutic Advances in Chronic Disease, p 15
Udrea AM et al (2023) Photosensitizers-loaded nanocarriers for enhancement of photodynamic therapy in Melanoma Treatment. Pharmaceutics, 15(8)
Huang Y, Hong W, Wei X (2022) The molecular mechanisms and therapeutic strategies of EMT in tumor progression and metastasis. J Hematol Oncol, 15(1)
LeBleu VS, Thiery JP (2022) The Continuing search for causality between epithelial-to-mesenchymal transition and the Metastatic Fitness of Carcinoma Cells. Cancer Res 82(8):1467–1469
doi: 10.1158/0008-5472.CAN-22-0026
pubmed: 35425957
Cheng X et al (2022) Insight into the prospects for Tumor Therapy based on photodynamic immunotherapy. Pharmaceuticals, 15(11)
Yu Q et al (2023) Recent advances in Reprogramming Strategy of Tumor Microenvironment for Rejuvenating Photosensitizers-mediated photodynamic therapy. Small, 20(16)
Dotto GP, Anil K, Rustgi (2016) Squamous cell cancers: a unified perspective on Biology and Genetics. Cancer Cell 29(5):622–637
doi: 10.1016/j.ccell.2016.04.004
pubmed: 27165741
pmcid: 4870309
Friedlaender A et al (2019) Next generation sequencing and genetic alterations in squamous cell lung carcinoma. Where Are We Today? Frontiers in Oncology, p 9
Hirsch FR et al (2017) Lung cancer: current therapies and new targeted treatments. Lancet 389(10066):299–311
doi: 10.1016/S0140-6736(16)30958-8
pubmed: 27574741
Socinski MA et al (2018) Current and Emergent Therapy options for Advanced squamous cell Lung Cancer. J Thorac Oncol 13(2):165–183
doi: 10.1016/j.jtho.2017.11.111
pubmed: 29175116
Li X et al (2020) Clinical development and potential of photothermal and photodynamic therapies for cancer. Nat Reviews Clin Oncol 17(11):657–674
doi: 10.1038/s41571-020-0410-2
Akopov A, Papayan G (2021) Photodynamic theranostics of central lung cancer: Present state and future prospects. Photodiagnosis Photodyn Ther 33:102203
doi: 10.1016/j.pdpdt.2021.102203
pubmed: 33529744
Chhatre S et al (2021) Survival outcomes with photodynamic therapy, Chemotherapy and Radiation in patients with Stage III or Stage IV Non-small Cell Lung Cancer. Cancers (Basel), 13(4)
Lin C et al (2021) Analysis of the short-term effect of photodynamic therapy on primary bronchial lung cancer. Lasers Med Sci 36(4):753–761
doi: 10.1007/s10103-020-03080-5
pubmed: 32594348
Du Y et al (2022) Glutathione depletion and photosensitizer activation augments efficacy of tumor photodynamic immunotherapy. Chem Eng J 442:136170
doi: 10.1016/j.cej.2022.136170
Heo S-Y et al (2023) Anti-cancer effect of Chlorophyllin-assisted photodynamic therapy to Induce apoptosis through oxidative stress on human cervical Cancer. Int J Mol Sci, 24(14)
Hu J, Wen X, Song J (2024) Hypericin-mediated photodynamic therapy inhibits metastasis and EMT of colorectal cancer cells by regulating RhoA–ROCK1 signaling pathway. Photochem Photobiol Sci 23(7):1361–1372
doi: 10.1007/s43630-024-00601-x
pubmed: 38865066
Jia Y et al (2019) Photodynamic therapy combined with temozolomide inhibits C6 glioma migration and invasion and promotes mitochondrial-associated apoptosis by inhibiting sodium-hydrogen exchanger isoform 1. Photodiagn Photodyn Ther 26:405–412
doi: 10.1016/j.pdpdt.2019.05.007
Lin C et al (2023) Effect of a 630 nm light on vasculogenic mimicry in A549 lung adenocarcinoma cells in vitro. Photodiagn Photodyn Ther 44:103831
doi: 10.1016/j.pdpdt.2023.103831
Qi S et al (2019) Hypocrellin A-based photodynamic action induces apoptosis in A549 cells through ROS-mediated mitochondrial signaling pathway. Acta Pharm Sinica B 9(2):279–293
doi: 10.1016/j.apsb.2018.12.004
Suzuki T et al (2020) Vascular shutdown by photodynamic therapy using Talaporfin Sodium. Cancers, 12(9)
Wang W et al (2023) Intratibial osteosarcoma growth and pulmonary metastasis inhibition by HIPPO signaling pathway-induced photodynamic therapy. Mater Design 233:112269
doi: 10.1016/j.matdes.2023.112269
Yao Y et al (2023) Elsinochrome a induces cell apoptosis and autophagy in photodynamic therapy. J Cell Biochem 124(9):1346–1365
doi: 10.1002/jcb.30451
pubmed: 37555580
Ye T et al (2020) 5-aminolevulinic acid photodynamic therapy inhibits invasion and metastasis of SCL-1 cells probably via MTSS1 and p63 gene related pathways. Photodiagn Photodyn Ther 32:102039
doi: 10.1016/j.pdpdt.2020.102039
Zhang T et al (2024) Synergistic effects of photodynamic therapy and chemotherapy: activating the intrinsic/extrinsic apoptotic pathway of anoikis for triple-negative breast cancer treatment. Biomaterials Adv 160:213859
doi: 10.1016/j.bioadv.2024.213859
Zheng L et al (2024) A novel photosensitizer DTPP-mediated photodynamic therapy induces oxidative stress and apoptosis through mitochondrial pathways in LA795 cells. Photodiagn Photodyn Ther 45:103894
doi: 10.1016/j.pdpdt.2023.103894
Ashkenazi A et al (2017) From basic apoptosis discoveries to advanced selective BCL-2 family inhibitors. Nat Rev Drug Discov 16(4):273–284
doi: 10.1038/nrd.2016.253
pubmed: 28209992
Delbridge AR et al (2016) Thirty years of BCL-2: translating cell death discoveries into novel cancer therapies. Nat Rev Cancer 16(2):99–109
doi: 10.1038/nrc.2015.17
pubmed: 26822577
Sun PL, Sasano H, Gao H (2017) Bcl-2 family in non-small cell lung cancer: its prognostic and therapeutic implications. Pathol Int 67(3):121–130
doi: 10.1111/pin.12507
pubmed: 28102575
Green DR (2022) Caspase activation and inhibition. Cold Spring Harb Perspect Biol, 14(8)
Singh R, Letai A, Sarosiek K (2019) Regulation of apoptosis in health and disease: the balancing act of BCL-2 family proteins. Nat Rev Mol Cell Biol 20(3):175–193
doi: 10.1038/s41580-018-0089-8
pubmed: 30655609
pmcid: 7325303
Liu G et al (2017) Role of Autophagy and apoptosis in Non-small-cell Lung Cancer. Int J Mol Sci, 18(2)
Sauler M, Bazan IS, Lee PJ (2019) Cell death in the lung: the apoptosis-necroptosis Axis. Annu Rev Physiol 81:375–402
doi: 10.1146/annurev-physiol-020518-114320
pubmed: 30485762
An HK et al (2020) CASP9 (caspase 9) is essential for autophagosome maturation through regulation of mitochondrial homeostasis. Autophagy 16(9):1598–1617
doi: 10.1080/15548627.2019.1695398
pubmed: 31818185
Li P et al (2017) Caspase-9: structure, mechanisms and clinical application. Oncotarget 8(14):23996–24008
doi: 10.18632/oncotarget.15098
pubmed: 28177918
pmcid: 5410359
Debnath P et al (2022) Epithelial–mesenchymal transition and its transcription factors. Biosci Rep, 42(1)
Yeung KT, Yang J (2016) Epithelial–mesenchymal transition in tumor metastasis. Mol Oncol 11(1):28–39
doi: 10.1002/1878-0261.12017
pubmed: 28085222
pmcid: 5242415
Sreter KB et al (2024) Resistance to KRAS inhibition in advanced non-small cell lung cancer. Front Oncol, 14
Hsieh K et al (2023) Radiotherapy and Immunotherapy in Lung Cancer. Biomedicines, 11(6)
Li J, Kwok H (2020) Current strategies for treating NSCLC: from Biological mechanisms to Clinical Treatment. Cancers, 12(6)
Zhang H et al (2024) Clinical characteristics and novel strategies of immune checkpoint inhibitor rechallenge therapy for non-small cell lung cancer: a comprehensive review. Front Immunol, 14
Brabletz S et al (2021) Dynamic EMT: a multi-tool for tumor progression. Embo j 40(18):e108647
doi: 10.15252/embj.2021108647
pubmed: 34459003
pmcid: 8441439
Shao L et al (2022) Effects of Curcumin-mediated photodynamic therapy on autophagy and epithelial-mesenchymal transition of lung cancer cells. Photodiagnosis Photodyn Ther 38:102849
doi: 10.1016/j.pdpdt.2022.102849
pubmed: 35390521
Musani AI et al (2018) Photodynamic therapy via navigational bronchoscopy for peripheral lung cancer in dogs. Lasers Surg Med 50(5):483–490
doi: 10.1002/lsm.22781
pubmed: 29399826
Sato H et al (2022) Combination of photodynamic therapy and endoscopic mucosal resection for recurrent esophageal squamous cell carcinoma after chemoradiotherapy. Clin J Gastroenterol 15(6):1035–1040
doi: 10.1007/s12328-022-01695-5
pubmed: 36064946
Tamura K et al (2023) Mucoepidermoid carcinoma cured by a combination of high-frequency snare and photodynamic therapy: a case report. Thorac Cancer 14(14):1306–1310
doi: 10.1111/1759-7714.14861
pubmed: 36929314
pmcid: 10175029