Transcriptional activation domains interact with ATPase subunits of yeast chromatin remodelling complexes SWI/SNF, RSC and INO80.

Saccharomyces cerevisiae Proteins / genetics Adenosine Triphosphatases / genetics Transcription Factors / genetics Saccharomyces cerevisiae / genetics Chromatin Assembly and Disassembly / genetics DNA-Binding Proteins / genetics Transcriptional Activation Protein Binding Chromosomal Proteins, Non-Histone / genetics Gene Expression Regulation, Fungal Protein Domains Nuclear Proteins Cell Cycle Proteins Basic Helix-Loop-Helix Transcription Factors
Saccharomyces cerevisiae Activation domains Activator binding domain Chromatin remodelling complex Ino80 Sth1 Swi2

Journal

Current genetics
ISSN: 1432-0983
Titre abrégé: Curr Genet
Pays: United States
ID NLM: 8004904

Informations de publication

Date de publication:
05 Sep 2024
Historique:
received: 11 06 2024
accepted: 07 08 2024
revised: 25 07 2024
medline: 5 9 2024
pubmed: 5 9 2024
entrez: 5 9 2024
Statut: epublish

Résumé

Chromatin remodelling complexes (CRC) are ATP-dependent molecular machines important for the dynamic organization of nucleosomes along eukaryotic DNA. CRCs SWI/SNF, RSC and INO80 can move positioned nucleosomes in promoter DNA, leading to nucleosome-depleted regions which facilitate access of general transcription factors. This function is strongly supported by transcriptional activators being able to interact with subunits of various CRCs. In this work we show that SWI/SNF subunits Swi1, Swi2, Snf5 and Snf6 can bind to activation domains of Ino2 required for expression of phospholipid biosynthetic genes in yeast. We identify an activator binding domain (ABD) of ATPase Swi2 and show that this ABD is functionally dispensable, presumably because ABDs of other SWI/SNF subunits can compensate for the loss. In contrast, mutational characterization of the ABD of the Swi2-related ATPase Sth1 revealed that some conserved basic and hydrophobic amino acids within this domain are essential for the function of Sth1. While ABDs of Swi2 and Sth1 define separate functional protein domains, mapping of an ABD within ATPase Ino80 showed co-localization with its HSA domain also required for binding actin-related proteins. Comparative interaction studies finally demonstrated that several unrelated activators each exhibit a specific binding pattern with ABDs of Swi2, Sth1 and Ino80.

Identifiants

DOI: 10.1007/s00294-024-01300-x PMID: 39235627
pubmed: 39235627
doi: 10.1007/s00294-024-01300-x
pii: 10.1007/s00294-024-01300-x
doi:

Substances chimiques

Saccharomyces cerevisiae Proteins 0
Adenosine Triphosphatases EC 3.6.1.-
Transcription Factors 0
DNA-Binding Proteins 0
INO80 complex, S cerevisiae 0
Chromosomal Proteins, Non-Histone 0
RSC complex, S cerevisiae 0
STH1 protein, S cerevisiae EC 3.6.1.-
INO2 protein, S cerevisiae 0
SNF2 protein, S cerevisiae EC 3.6.1.-
SWI1 protein, S cerevisiae 0
Nuclear Proteins 0
Cell Cycle Proteins 0
Basic Helix-Loop-Helix Transcription Factors 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

15

Informations de copyright

© 2024. The Author(s).

Références

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Auteurs

Eva-Carina Wendegatz (EC)

Center for Functional Genomics of Microbes, Institut Für Genetik Und Funktionelle Genomforschung, Universität Greifswald, Felix-Hausdorff-Strasse 8, 17487, Greifswald, Germany.

Maike Engelhardt (M)

Center for Functional Genomics of Microbes, Institut Für Genetik Und Funktionelle Genomforschung, Universität Greifswald, Felix-Hausdorff-Strasse 8, 17487, Greifswald, Germany.
Cheplapharm, Greifswald, Germany.

Hans-Joachim Schüller (HJ)

Center for Functional Genomics of Microbes, Institut Für Genetik Und Funktionelle Genomforschung, Universität Greifswald, Felix-Hausdorff-Strasse 8, 17487, Greifswald, Germany. schuell@uni-greifswald.de.
ORCID: 0000-0003-4080-4014

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines fongiques › Protéines de Saccharomyces cerevisiae › Transcriptional activation domains interact...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Acid anhydride hydrolases › Adenosine triphosphatases › Transcriptional activation domains interact...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Transcriptional activation domains interact...
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Saccharomyces › Saccharomyces cerevisiae › Transcriptional activation domains interact...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Assemblage et désassemblage de la chromatine › Transcriptional activation domains interact...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de liaison à l'ADN › Transcriptional activation domains interact...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Activation de la transcription › Transcriptional activation domains interact...
questionsmedicales.fr › Métabolisme › Liaison aux protéines › Transcriptional activation domains interact...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Protéines chromosomiques nonhistones › Transcriptional activation domains interact...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes fongiques › Transcriptional activation domains interact...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure tertiaire des protéines › Domaines protéiques › Transcriptional activation domains interact...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines nucléaires › Transcriptional activation domains interact...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du cycle cellulaire › Transcriptional activation domains interact...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteurs de transcription à motif basique hélice-boucle-hélice › Transcriptional activation domains interact...