Diarrhoeagenic Escherichia coli associated with childhood diarrhoea in Osun state, Nigeria.
Humans
Nigeria
/ epidemiology
Diarrhea
/ microbiology
Infant
Female
Child, Preschool
Male
Escherichia coli Infections
/ microbiology
Escherichia coli
/ genetics
Anti-Bacterial Agents
/ pharmacology
Feces
/ microbiology
Microbial Sensitivity Tests
Infant, Newborn
Risk Factors
Drug Resistance, Bacterial
Antimicrobial resistance
Diarrhoea
Diarrhoeagenic Escherichia coli
Journal
BMC infectious diseases
ISSN: 1471-2334
Titre abrégé: BMC Infect Dis
Pays: England
ID NLM: 100968551
Informations de publication
Date de publication:
08 Sep 2024
08 Sep 2024
Historique:
received:
25
04
2024
accepted:
22
08
2024
medline:
9
9
2024
pubmed:
9
9
2024
entrez:
8
9
2024
Statut:
epublish
Résumé
Diarrhoea is a major public health concern in developing countries, usually exacerbated by poor water, sanitation and hygiene but its aetiology is under-studied, particularly away from capital cities. We identified diarrhoeagenic Escherichia coli (DEC) from stools collected in Ile-Ife and Ilesa, Osun state, Nigeria and determined their antibiotic resistance profiles. Stool samples from 167 children with diarrhoea and 334 controls under the age of 5 years were cultured for Escherichia coli and Salmonella. Bacterial isolates were identified biochemically and DEC were identified by PCR. Antimicrobial susceptibility testing was by modified Kirby-Bauer disc diffusion method in accordance with the CLSI guidelines. Data were analyzed using Chi-square and Fisher's exact tests. Diarrhoea infection is significantly high among children under 12 months (p = 0.002), caregivers without at least primary school education (p = 0.006), breastfeeding for under 6 months (p˂0.001), and caregivers who were siblings (p = 0.004). DEC was detected in 69(41.3%) cases but only 86(25.7%) controls (p < 0.001) and more commonly recovered during the wet season (p < 0.001). Enterotoxigenic E. coli (p = 0.031), enteropathogenic E. coli (p = 0.031) and Shiga-toxin-producing E. coli (p = 0.044) were recovered more commonly from cases than controls. DEC from patients with diarrhoea were commonly resistant to sulphonamides (91.3%), trimethoprim (82.6%), and ampicillin (78.3%) but were largely susceptible to quinolones and carbapenems (97.1%). Enteropathogenic, enterotoxigenic and Shiga toxin-producing E. coli are associated with diarrhoea in our setting, and show considerable resistance to first-line antimicrobials. Risk factors for DEC diarrhoea include infancy, inadequate breastfeeding and caregivers with education below primary school.
Identifiants
pubmed: 39245731
doi: 10.1186/s12879-024-09793-0
pii: 10.1186/s12879-024-09793-0
doi:
Substances chimiques
Anti-Bacterial Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
928Subventions
Organisme : African Research Leader's Award
ID : MR/L00464X/1
Organisme : Bill & Melinda Gates Foundation
ID : INV-036234
Pays : United States
Informations de copyright
© 2024. The Author(s).
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