Genetic diversity and epidemiological insights into cutaneous leishmaniasis in Pakistan: a comprehensive study on clinical manifestations and molecular characterization of Leishmania species.
Leishmaniasis, Cutaneous
/ epidemiology
Pakistan
/ epidemiology
Humans
Genetic Variation
Male
Adolescent
Adult
Female
Young Adult
Child
Middle Aged
Leishmania
/ genetics
Child, Preschool
Sequence Analysis, DNA
Leishmania tropica
/ genetics
Leishmania major
/ genetics
DNA, Protozoan
/ genetics
Phylogeny
Molecular Epidemiology
Aged
Real-Time Polymerase Chain Reaction
Leishmania spp.
Cutaneous leishmaniasis
ITS-1
Molecular characterization
Pakistan
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
10 Sep 2024
10 Sep 2024
Historique:
received:
21
02
2024
accepted:
03
09
2024
medline:
10
9
2024
pubmed:
10
9
2024
entrez:
10
9
2024
Statut:
epublish
Résumé
Cutaneous leishmaniasis (CL) stands out as a significant vector-borne endemic in Pakistan. Despite the rising incidence of CL, the genetic diversity of Leishmania species in the country's endemic regions remains insufficiently explored. This study aims to uncover the genetic diversity and molecular characteristics of Leishmania species in CL-endemic areas of Baluchistan, Khyber Pakhtunkhwa (KPK), and Punjab in Pakistan. Clinical samples from 300 CL patients were put to microscopic examination, real-time ITS-1 PCR, and sequencing. Predominantly affecting males between 16 to 30 years of age, with lesions primarily on hands and faces, the majority presented with nodular and plaque types. Microscopic analysis revealed a positivity rate of 67.8%, while real-time PCR identified 60.98% positive cases, mainly L. tropica, followed by L. infantum and L. major. Leishmania major (p = 0.009) showed substantially greater variation in nucleotide sequences than L. tropica (p = 0.07) and L. infantum (p = 0.03). Nucleotide diversity analysis indicated higher diversity in L. major and L. infantum compared to L. tropica. This study enhances our understanding of CL epidemiology in Pakistan, stressing the crucial role of molecular techniques in accurate species identification. The foundational data provided here emphasizes the necessity for future research to investigate deeper into genetic diversity and its implications for CL control at both individual and community levels.
Identifiants
pubmed: 39254766
doi: 10.1007/s00436-024-08344-5
pii: 10.1007/s00436-024-08344-5
doi:
Substances chimiques
DNA, Protozoan
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
320Subventions
Organisme : The Scientific and Technical Research Council of Turkey and Pakistan Science Foundation
ID : Project No: 118S658 and Project No. PSF TUBITAK/Med /P-NUMS(09)
Organisme : The Scientific and Technical Research Council of Turkey and Pakistan Science Foundation
ID : Project No: 118S658 and Project No. PSF TUBITAK/Med /P-NUMS(09)
Organisme : The Scientific and Technical Research Council of Turkey and Pakistan Science Foundation
ID : Project No: 118S658 and Project No. PSF TUBITAK/Med /P-NUMS(09)
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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