Equilibrium dialysis with HPLC detection to measure substrate binding affinity of a non-heme iron halogenase.

Chromatography, High Pressure Liquid / methods Substrate Specificity Dialysis / methods Protein Binding Enzyme Assays / methods Kinetics Lysine / metabolism Oxidoreductases / metabolism Bacterial Proteins / metabolism Iron / metabolism
AQC derivatization Amino acid detection Biocatalysts Equilibrium dialysis Non-heme iron halogenases

Journal

Methods in enzymology
ISSN: 1557-7988
Titre abrégé: Methods Enzymol
Pays: United States
ID NLM: 0212271

Informations de publication

Date de publication:
2024
Historique:
medline: 12 9 2024
pubmed: 12 9 2024
entrez: 11 9 2024
Statut: ppublish

Résumé

Determination of substrate binding affinity (K

Identifiants

DOI: 10.1016/bs.mie.2024.05.004 PMID: 39261003
pubmed: 39261003
pii: S0076-6879(24)00194-0
doi: 10.1016/bs.mie.2024.05.004
pii:
doi:

Substances chimiques

Lysine K3Z4F929H6
Oxidoreductases EC 1.-
Bacterial Proteins 0
Iron E1UOL152H7

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

51-63

Informations de copyright

Copyright © 2024. Published by Elsevier Inc.

Auteurs

Elizabeth R Smithwick (ER)

Department of Chemistry, University of Minnesota - Twin Cities, Minneapolis, MN, United States.

Ambika Bhagi-Damodaran (A)

Department of Chemistry, University of Minnesota - Twin Cities, Minneapolis, MN, United States. Electronic address: ambikab@umn.edu.

Anoop Rama Damodaran (AR)

Department of Chemistry, University of Minnesota - Twin Cities, Minneapolis, MN, United States. Electronic address: rdanoop@umn.edu.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie en phase liquide à haute performance › Equilibrium dialysis with HPLC detection to...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Spécificité du substrat › Equilibrium dialysis with HPLC detection to...
questionsmedicales.fr › Phénomènes chimiques › Dialyse › Equilibrium dialysis with HPLC detection to...
questionsmedicales.fr › Métabolisme › Liaison aux protéines › Equilibrium dialysis with HPLC detection to...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Dosages enzymatiques › Equilibrium dialysis with HPLC detection to...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › Equilibrium dialysis with HPLC detection to...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés essentiels › Lysine › Equilibrium dialysis with HPLC detection to...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Equilibrium dialysis with HPLC detection to...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Equilibrium dialysis with HPLC detection to...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Métaux lourds › Fer › Equilibrium dialysis with HPLC detection to...