Rapid analysis of amatoxins in human urine by means of affinity column chromatography and liquid chromatography-high-resolution tandem mass spectrometry.
Affinity column chromatography
Amatoxins
Liquid chromatography-high-resolution mass spectrometry
Magnetic beads
Method development
Monoclonal antibody
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
13 Sep 2024
13 Sep 2024
Historique:
received:
09
07
2024
accepted:
08
09
2024
medline:
14
9
2024
pubmed:
14
9
2024
entrez:
13
9
2024
Statut:
epublish
Résumé
Analysis of amatoxins is of great importance as these cyclic peptides contribute to a high number of fatalities each year. Development of analytical approaches needs to focus on rapid, sensitive, and reliable methods. By establishing an affinity column chromatography-based assay using the monoclonal amanitin antibody AMA9G3 and liquid chromatography (LC) coupled to high-resolution mass spectrometry (HRMS) for the trace detection of α-, β-, and γ-amanitin in human urine samples to confirm ingestion, we report the first approach that extents the current status of amatoxin analysis. The presented procedure allows detection of amatoxins in human urine down to 1 ng/mL. The method was successfully validated qualitatively for α- and γ-amanitin according to international recommendations. A proof of concept was performed by analyzing 37 urine samples after suspected amatoxin consumption submitted for regular clinical toxicological analysis. Using this antibody-based enrichment strategy, acute amatoxin intoxications can be determined within 90 min and due to the high sensitivity and selectivity, a comparable approach using target specific antibodies may also be used for other toxicological relevant peptides.
Identifiants
pubmed: 39271810
doi: 10.1038/s41598-024-72463-3
pii: 10.1038/s41598-024-72463-3
doi:
Substances chimiques
Amanitins
0
amatoxin
58250-15-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
21397Subventions
Organisme : U.S. Department of Agriculture
ID : CRIS 2030-42000-053-000-D
Informations de copyright
© 2024. The Author(s).
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