Rate of hydrolysis of the phosphate esters of B vitamins is reduced by zinc deficiency: In vitro and in vivo.
B vitamins (vitamins B1, B2, and B6)
ectoenzyme
hydrolysis
phosphate ester
zinc deficiency
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484
Informations de publication
Date de publication:
30 Sep 2024
30 Sep 2024
Historique:
revised:
05
08
2024
received:
04
06
2024
accepted:
20
08
2024
medline:
16
9
2024
pubmed:
16
9
2024
entrez:
16
9
2024
Statut:
ppublish
Résumé
Extracellular hydrolysis of the phosphate esters of B vitamins (B1, B2, and B6) is crucial for their cellular uptake and metabolism. Although a few zinc-dependent enzymes have been implicated in these processes, their exact mechanisms of action remain largely unknown. This study investigated the potential involvement of phosphate group hydrolyzing enzymes in the hydrolysis of B vitamin phosphate esters. We evaluated enzyme activity in membrane lysates prepared from cells transiently transfected with these enzymes or those endogenously expressing them. Specifically, we investigated how zinc deficiency affects the rate of hydrolysis of B vitamin phosphate esters in cellular lysates. Assessment of the activities of zinc-dependent ectoenzymes in the lysates prepared from cells cultured in zinc-deficient conditions and in the serum of rats fed zinc-deficient diets revealed that zinc deficiency reduced the extracellular hydrolysis activity of B vitamin phosphate esters. Furthermore, our findings explain the similarities between several symptoms of B vitamin and zinc deficiencies. Collectively, this study provides novel insights into the diverse symptoms of zinc deficiency and could guide the development of appropriate clinical strategies.
Identifiants
pubmed: 39279493
doi: 10.1096/fj.202401207R
doi:
Substances chimiques
Zinc
J41CSQ7QDS
Esters
0
Vitamin B Complex
12001-76-2
Phosphates
0
Vitamin B 6
8059-24-3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e70025Subventions
Organisme : Ministry of Education, Culture, Sports, Science and Technology (MEXT)
ID : JP19H05768
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP19H02883
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP22H02257
Organisme : Sugiyama Chemical & Industrial Laboratory
Organisme : Kieikai Research Foundation
Organisme : Yakult Bio-Science Foundation (YBSF)
Organisme : Tojuro Iijima Foundation for Food Science and Technology
Organisme : KOSE Cosmetology Research Foundation
Organisme : Naito Foundation
Organisme : Japan Foundation for Applied Enzymology
Informations de copyright
© 2024 The Author(s). The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.
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