Freeze-Driven Adsorption of Oligonucleotides with polyA-Anchors on Au@Pt Nanozyme.

freeze–thaw method gold nanoparticles lateral flow test nanozymes oligonucleotide conjugation peroxidase-like activity platinum nanoparticles spherical nucleic acids

Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
20 Sep 2024
Historique:
received: 22 08 2024
revised: 15 09 2024
accepted: 18 09 2024
medline: 28 9 2024
pubmed: 28 9 2024
entrez: 28 9 2024
Statut: epublish

Résumé

A promising and sought-after class of nanozymes for various applications is Pt-containing nanozymes, primarily Au@Pt, due to their easy preparation and remarkable catalytic properties. This study aimed to explore the freeze-thaw method for functionalizing Pt-containing nanozymes with oligonucleotides featuring a polyadenine anchor. Spherical gold nanoparticles ([Au]NPs) were synthesized and subsequently used as seeds to produce urchin-like Au@Pt nanoparticles ([Au@Pt]NPs) with an average diameter of 29.8 nm. The nanoparticles were conjugated with a series of non-thiolated DNA oligonucleotides, each consisting of three parts: a 5'-polyadenine anchor (A

Identifiants

pubmed: 39337597
pii: ijms251810108
doi: 10.3390/ijms251810108
pii:
doi:

Substances chimiques

Gold 7440-57-5
Oligonucleotides 0
Platinum 49DFR088MY
Poly A 24937-83-5

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Ministry of Science and Higher Education of the Russian Federation
ID : 075-15-2022-318

Auteurs

Nikita E Lapshinov (NE)

A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.

Svetlana M Pridvorova (SM)

A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.

Anatoly V Zherdev (AV)

A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.

Boris B Dzantiev (BB)

A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.

Irina V Safenkova (IV)

A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.

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Classifications MeSH