A missense mutation in the barley Xan-h gene encoding the Mg-chelatase subunit I leads to a viable pale green line with reduced daily transpiration rate.
Barley
Canopy photosynthesis
Chlorophyll biosynthesis
Drought stress
Mg-chelatase
Pale green leaves
Journal
Plant cell reports
ISSN: 1432-203X
Titre abrégé: Plant Cell Rep
Pays: Germany
ID NLM: 9880970
Informations de publication
Date de publication:
29 Sep 2024
29 Sep 2024
Historique:
received:
04
05
2024
accepted:
12
09
2024
medline:
30
9
2024
pubmed:
30
9
2024
entrez:
29
9
2024
Statut:
epublish
Résumé
The barley mutant xan-h.chli-1 shows phenotypic features, such as reduced leaf chlorophyll content and daily transpiration rate, typical of wild barley accessions and landraces adapted to arid climatic conditions. The pale green trait, i.e. reduced chlorophyll content, has been shown to increase the efficiency of photosynthesis and biomass accumulation when photosynthetic microorganisms and tobacco plants are cultivated at high densities. Here, we assess the effects of reducing leaf chlorophyll content in barley by altering the chlorophyll biosynthesis pathway (CBP). To this end, we have isolated and characterised the pale green barley mutant xan-h.chli-1, which carries a missense mutation in the Xan-h gene for subunit I of Mg-chelatase (HvCHLI), the first enzyme in the CBP. Intriguingly, xan-h.chli-1 is the only known viable homozygous mutant at the Xan-h locus in barley. The Arg298Lys amino-acid substitution in the ATP-binding cleft causes a slight decrease in HvCHLI protein abundance and a marked reduction in Mg-chelatase activity. Under controlled growth conditions, mutant plants display reduced accumulation of antenna and photosystem core subunits, together with reduced photosystem II yield relative to wild-type under moderate illumination, and consistently higher than wild-type levels at high light intensities. Moreover, the reduced content of leaf chlorophyll is associated with a stable reduction in daily transpiration rate, and slight decreases in total biomass accumulation and water-use efficiency, reminiscent of phenotypic features of wild barley accessions and landraces that thrive under arid climatic conditions.
Identifiants
pubmed: 39343835
doi: 10.1007/s00299-024-03328-2
pii: 10.1007/s00299-024-03328-2
doi:
Substances chimiques
magnesium chelatase
EC 4.99.1-
Chlorophyll
1406-65-1
Plant Proteins
0
Lyases
EC 4.-
Photosystem II Protein Complex
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
246Subventions
Organisme : Next-Generation EU: PIANO NAZIONALE DI RIPRESA E RESILIENZA
ID : 1032 17/06/2022
Organisme : Next-Generation EU: PIANO NAZIONALE DI RIPRESA E RESILIENZA
ID : CN00000022
Organisme : Ministero degli Affari Esteri e della Cooperazione Internazionale
ID : Italy-Israel Joint Call for Proposals on Scientific
Organisme : Ministero degli Affari Esteri e della Cooperazione Internazionale
ID : Technological Cooperation 2021
Organisme : HORIZON EUROPE Food, Bioeconomy, Natural Resources, Agriculture and Environment
ID : 101082091 - BEST-CROP
Informations de copyright
© 2024. The Author(s).
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