Molecular detection and characterization of Trichomonas gallinae isolated from ornamental birds in Tehran, Iran.
Animals
Trichomonas
/ genetics
Iran
Trichomonas Infections
/ parasitology
Bird Diseases
/ parasitology
DNA, Ribosomal Spacer
/ genetics
DNA, Protozoan
/ genetics
Phylogeny
RNA, Ribosomal, 5.8S
/ genetics
Genetic Variation
Sequence Analysis, DNA
Columbidae
/ parasitology
Iron-Sulfur Proteins
/ genetics
Polymerase Chain Reaction
Hydrogenase
/ genetics
Prevalence
Finches
/ parasitology
Birds
/ parasitology
Cluster Analysis
Microscopy
Trichomonas gallinae
Avian host
DNA extraction
Molecular analysis
PCR
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
30 Sep 2024
30 Sep 2024
Historique:
received:
11
05
2024
accepted:
04
09
2024
medline:
30
9
2024
pubmed:
30
9
2024
entrez:
30
9
2024
Statut:
epublish
Résumé
Trichomonas gallinae is a widespread protozoan parasite that primarily affects birds, causing a disease known as avian trichomonosis. The present study aimed to investigate the prevalence and genetic diversity of T. gallinae, a parasite causing avian trichomoniasis in feral pigeons, budgerigars, and finches in Tehran, Iran. The 5.8S ribosomal RNA locus, along with the internal transcribed spacer 2 (ITS2) region, has been extensively utilized for genotype identification and for determining inter- and intra-specific diversity. More recently, the Fe-hydrogenase (Fe-Hyd) gene has been suggested as an additional genetic marker to enhance the accuracy of strain subtyping discrimination. In the present study, a total of 12% (12/100) birds examined were infected with T. gallinae using microscopy and PCR methods. Infection was found in seven of 30 (23.3%) feral pigeons, three of 40 (7.5%) budgerigars, and two of 30 (6.66%) finches. Analysis of the ITS2 region of T. gallinae isolates revealed two highly similar sequences. The first sequence (GenBank: OQ689964-OQ689970) was found in five feral pigeons and two budgerigars, whereas the second sequence (GenBank: OQ689971-OQ689975) was identified in two feral pigeons, one budgerigar, and two finches. Phylogenetic analysis confirmed the presence of two distinct clusters (cluster I and cluster II) within the trichomonads based on the ITS2 region. However, further analysis using Fe-Hyd revealed greater diversity, with three subtypes identified (A1, A2, and C1). One isolate identified in the present study (GenBank accession number: OQ694508.1) belonged to subtype A1. Combining ITS2 and Fe-Hyd markers holds promise for a more comprehensive understanding of the population structure of T. gallinae and the potential role of ITS2 in host adaptation.
Identifiants
pubmed: 39347844
doi: 10.1007/s00436-024-08346-3
pii: 10.1007/s00436-024-08346-3
doi:
Substances chimiques
DNA, Ribosomal Spacer
0
DNA, Protozoan
0
RNA, Ribosomal, 5.8S
0
iron hydrogenase
EC 1.12.-
Iron-Sulfur Proteins
0
Hydrogenase
EC 1.12.7.2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
335Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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