Genomic analysis of Enterobacteriaceae from colorectal cancer patients at a tertiary hospital in Ghana: a case-control study.
Humans
Ghana
/ epidemiology
Colorectal Neoplasms
/ microbiology
Female
Male
Middle Aged
Case-Control Studies
Tertiary Care Centers
Enterobacteriaceae
/ genetics
Aged
Escherichia coli
/ genetics
Whole Genome Sequencing
Genome, Bacterial
Adult
Enterobacteriaceae Infections
/ microbiology
Virulence Factors
/ genetics
Genomics
/ methods
Enterobacteriaceae
Antimicrobial resistance
Colorectal cancer
Genomics
Virulence factors
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
05 Oct 2024
05 Oct 2024
Historique:
received:
12
07
2024
accepted:
25
09
2024
medline:
6
10
2024
pubmed:
6
10
2024
entrez:
5
10
2024
Statut:
epublish
Résumé
Colorectal cancer (CRC) is a severe gastrointestinal cancer and a leading cause of cancer-related deaths in Ghana. The potential role of gut Enterobacteriaceae in the increasing incidence of CRC in Ghana is yet to be thoroughly investigated. In this study, Enterobacteriaceae from CRC patients and healthy control participants were analyzed by whole genome sequencing to identify genomic features that are associated with CRC. Socio-demographic data showed a significant association between age and alcohol consumption and CRC. Escherichia coli was the most abundant Enterobacteriaceae isolated from the study participants and they were predominantly intestinal commensals. Escherichia coli isolates belonging to phylogroup D encoded the highest number of virulence genes. The agn43 and int genes were widespread in Escherichia coli isolates from the CRC patients. Multilocus sequence types of potentially pathogenic Escherichia coli from the CRC patients also encoded genes involved in aggregation, adherence and biofilm formation. The ampC2 and ampH antimicrobial resistance genes were also widespread in the genome of the Escherichia coli isolates. This study highlights the virulence tendencies of Escherichia coli from CRC patients and their ability to transfer virulence determinants to other Enterobacteriaceae residing in the gut.
Identifiants
pubmed: 39369124
doi: 10.1038/s41598-024-74299-3
pii: 10.1038/s41598-024-74299-3
doi:
Substances chimiques
Virulence Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
23195Subventions
Organisme : BANGA-AFRICA PROJECT; UNIVERSITY OF GHANA
ID : UG-BA/SRG-011/2022
Informations de copyright
© 2024. The Author(s).
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