Occurrence of Extended-spectrum β-lactamase (ESBL) and Carbapenemase-producing Escherichia coli isolated from Childhood Diarrhoea in Yaoundé, Cameroon.
Humans
beta-Lactamases
/ genetics
Cameroon
/ epidemiology
Diarrhea
/ microbiology
Escherichia coli Infections
/ microbiology
Child, Preschool
Escherichia coli
/ genetics
Female
Infant
Male
Microbial Sensitivity Tests
Anti-Bacterial Agents
/ pharmacology
Feces
/ microbiology
Bacterial Proteins
/ genetics
Virulence Factors
/ genetics
Drug Resistance, Multiple, Bacterial
/ genetics
Antibiotic resistance
Cameroon
Children
ESBLs
Multidrug resistance
Journal
BMC microbiology
ISSN: 1471-2180
Titre abrégé: BMC Microbiol
Pays: England
ID NLM: 100966981
Informations de publication
Date de publication:
10 Oct 2024
10 Oct 2024
Historique:
received:
21
05
2024
accepted:
30
09
2024
medline:
10
10
2024
pubmed:
10
10
2024
entrez:
9
10
2024
Statut:
epublish
Résumé
Extended-spectrum β-lactamase (ESBL)-producing pathogenic E. coli is a global public health issue, especially in sub-Saharan African countries such as Cameroon. It contributes to increase significantly hospital length of stay, morbidity, mortality and economic costs because of treatment failures. This study aims at determining the resistance background and virulence profiles of ESBL-E. coli isolates among childhood diarrhoea during the cholera outbreak occuring in Yaoundé, Cameroon. During a four-month periods, from March 1st to June 30th, 2023, a total of 84 stool samples were collected from 90 under five children presenting clinical signs of gastroenteritis and attending four hospitals in Yaoundé, Cameroon. Bacterial identification was done using API20E and antimicrobial susceptibility test was performed using the Kirby-Bauer disc diffusion method. After extraction, genomic DNA was subjected to conventional and multiplex polymerase chain reaction methods (PCRs) for detection of resistance and virulence genes. Statistical analysis was performed using Epi info™ (7.2.5.0). Statistical significance was considered at a p-value < 0.05. Out of 150 patients contacted, 90 patients were enrolled, 84 samples were collected, 52.38%(44/84) and 3.57%(03/84) were confirmed as extended-spectrum β-lactamase and carbapenemase-producing E. coli respectively. The risk factors were analyzed, and children who drank natural fruit juice (OR: 0.4, p-value: 0.03) were found to be significantly associated with ESBL-producing E. coli. The ESBL-producing E. coli isolates showed a high level of resistance to amoxicillin-clavulanic acid, cefotaxime, ceftazidime, cefepime, colistin, and tetracycline. The bla These findings suggest implementing routine surveillance and screening for antimicrobial resistance among children under five. Antimicrobial stewardship strategies (ASP) need to be implemented to curb the emergence and dissemination of ESBL-producing E. coli. In addition, a national surveillance program for antimicrobial resistance needs to be implemented at local and regional levels in order to reduce morbidity in Cameroon.
Identifiants
pubmed: 39385062
doi: 10.1186/s12866-024-03559-z
pii: 10.1186/s12866-024-03559-z
doi:
Substances chimiques
beta-Lactamases
EC 3.5.2.6
Anti-Bacterial Agents
0
carbapenemase
EC 3.5.2.6
Bacterial Proteins
0
Virulence Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
401Subventions
Organisme : Thrasher Early Career Award
ID : 01364
Informations de copyright
© 2024. The Author(s).
Références
Tola MA, Abera NA, Gebeyehu YM, Dinku SF, Tullu KD. High prevalence of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae fecal carriage among children under five years in Addis Ababa, Ethiopia. PLoS ONE. 2021;16(10):e0258117.
doi: 10.1371/journal.pone.0258117
Torgerson PR, Devleesschauwer B, Praet N, Speybroeck N, Willingham AL, Kasuga F, et al. World Health Organization Estimates of the Global and Regional Disease Burden of 11 foodborne parasitic diseases, 2010: A Data Synthesis. PLoS Med. 2015;12(12):e1001920.
doi: 10.1371/journal.pmed.1001920
Murray CJL, Ikuta KS, Sharara F, Swetschinski L, Robles Aguilar G, Gray A, et al. Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis. Lancet. 2022;399(10325):629–55.
doi: 10.1016/S0140-6736(21)02724-0
Afum T, Asandem DA, Asare P, Asante-Poku A, Mensah GI, Musah AB, et al. Diarrhea-causing Bacteria and their antibiotic resistance patterns among Diarrhea patients from Ghana. Front Microbiol. 2022;13:894319.
doi: 10.3389/fmicb.2022.894319
Kirk MD, Pires SM, Black RE, Caipo M, Crump JA, Devleesschauwer B, et al. World Health Organization Estimates of the Global and Regional Disease Burden of 22 foodborne bacterial, Protozoal, and viral diseases, 2010: A Data Synthesis. PLoS Med. 2015;12(12):e1001921.
doi: 10.1371/journal.pmed.1001921
Dela H, Egyir B, Majekodunmi AO, Behene E, Yeboah C, Ackah D, et al. Diarrhoeagenic E. Coli occurrence and antimicrobial resistance of Extended Spectrum Beta-Lactamases isolated from diarrhoea patients attending health facilities in Accra, Ghana. PLoS ONE. 2022;17(5):e0268991.
doi: 10.1371/journal.pone.0268991
Lee W, Sung S, Ha J, Kim E, An ES, Kim SH et al. Molecular and genomic analysis of the virulence factors and potential transmission of Hybrid Enteropathogenic and Enterotoxigenic Escherichia coli (EPEC/ETEC) strains isolated in South Korea. Int J Mol Sci. 2023;24(16).
Saka HK, García-Soto S, Dabo NT, Lopez-Chavarrias V, Muhammad B, Ugarte-Ruiz M, et al. Molecular detection of extended spectrum β-lactamase genes in Escherichia coli clinical isolates from diarrhoeic children in Kano, Nigeria. PLoS ONE. 2020;15(12):e0243130.
doi: 10.1371/journal.pone.0243130
Amin M, Sirous M, Javaherizadeh H, Motamedifar M, Saki M, Veisi H, et al. Antibiotic resistance pattern and molecular characterization of extended-spectrum β-lactamase producing enteroaggregative Escherichia coli isolates in children from southwest Iran. Infect Drug Resist. 2018;11:1097–104.
doi: 10.2147/IDR.S167271
Zemtsa RJ, Noubom M, Founou LL, Dimani BD, Koudoum PL, Mbossi AD et al. Multidrug-Resistant and Extended-Spectrum β-Lactamase (ESBL) - Producing Enterobacterales Isolated from Carriage Samples among HIV Infected Women in Yaoundé, Cameroon. Pathogens. 2022;11(5).
Nkengkana OA, Founou RC, Founou LL, Dimani BD, Koudoum PL, Zemtsa JR, et al. Phenotypic and genotypic characterization of multidrug resistant and extended-spectrum β-lactamase-producing enterobacterales isolated from clinical samples in the western region in Cameroon. BMC Infect Dis. 2023;23(1):819.
doi: 10.1186/s12879-023-08742-7
Jérôme A, Landry BB, Etienne G, Anthony NC, Benjamin AC, André PGK, et al. Profil et antibiosensibilité des bactéries pathogènes associées aux diarrhées chez les patients consultant à l’Hôpital Régional Annexe De Kousseri, Extrême-Nord Cameroun. PAMJ. 2018;29:170.
CA-SFM. Comité de l’Antibiogramme de la Société Francaise de Microbiologie: Recommandations. 2023.
Dimani BD, Founou RC, Zemtsa JR, Mbossi A, Koudoum PL, Founou LL, et al. Faecal carriage of multidrug-resistant and extended-spectrum β-lactamase-producing enterobacterales in people living with HIV in Yaoundé, Cameroon. J Glob Antimicrob Resist. 2023;35:26–34.
doi: 10.1016/j.jgar.2023.07.021
Founou LL, Founou RC, Allam M, Ismail A, Essack SY. Genome Analysis of ESBL-Producing Escherichia coli Isolated from Pigs. Pathogens. 2022; 11(7).
Founou LL, Founou RC, Allam M, Ismail A, Essack SY. Extended-spectrum beta-lactamase-producing Escherichia coli harbouring mcr-1 gene isolated from pigs in South Africa. SAMJ. 2018;108(10):796–7.
doi: 10.7196/SAMJ.2018.v108i10.13508
Haghighatpanah M, Mozaffari Nejad AS, Mojtahedi A, Amirmozafari N, Zeighami H. Detection of extended-spectrum β-lactamase (ESBL) and plasmid-borne blaCTX-M and blaTEM genes among clinical strains of Escherichia coli isolated from patients in the north of Iran. J Global Antimicrob Resis. 2016;7:110–3.
doi: 10.1016/j.jgar.2016.08.005
Dembélé R, Konaté A, Traoré O, Kaboré WAD, Soulama I, Kagambèga A, et al. Extended spectrum beta-lactamase and fluoroquinolone resistance genes among Escherichia coli and Salmonella isolates from children with diarrhea, Burkina Faso. BMC Pediatr. 2020;20(1):459.
doi: 10.1186/s12887-020-02342-z
Tawfick MM, Elshamy AA, Mohamed KT, El Menofy NG. Gut commensal Escherichia coli, a High-Risk Reservoir of Transferable plasmid-mediated Antimicrobial Resistance traits. Infect Drug Resist. 2022;15:1077–91.
doi: 10.2147/IDR.S354884
Suge T, Magu D, Wanzala P. Antibiotic utilization and resistance in diarrheagenic Escherichia coli isolated from children under 5 years in Nakuru County: a case-control study. F1000Research. 2023;12(1072).
Eger E, Heiden SE, Korolew K, Bayingana C, Ndoli JM, Sendegeya A et al. Circulation of extended-spectrum beta-lactamase-producing Escherichia coli of Pandemic sequence types 131, 648, and 410 among hospitalized patients, caregivers, and the community in Rwanda. Front Microbiol. 2021;12.
Castanheira M, Simner PJ, Bradford PA. Extended-spectrum β-lactamases: an update on their characteristics, epidemiology and detection. JAC Antimicrob Resist. 2021;3(3):dlab092.
doi: 10.1093/jacamr/dlab092
Hussain HI, Iqbal Z, Iqbal M, Kuang X, Wang Y, Yang L, et al. Coexistence of virulence and β-lactamase genes in avian pathogenic Escherichia coli. Microb Pathogen. 2022;163:105389.
doi: 10.1016/j.micpath.2022.105389