Cases of endophthalmitis caused by Candida albicans and Candida dubliniensis identified via internal transcribed spacer deep sequencing.
Humans
Endophthalmitis
/ microbiology
Middle Aged
Female
Eye Infections, Fungal
/ microbiology
Male
Candidiasis
/ microbiology
High-Throughput Nucleotide Sequencing
Candida albicans
/ isolation & purification
Candida
/ genetics
DNA, Fungal
/ genetics
Vitrectomy
Antifungal Agents
/ therapeutic use
Vitreous Body
/ microbiology
Candida albicans
Candida dubliniensis
Deep sequencing
Endophthalmitis
Internal transcribed spacer
Metagenomic analysis
Journal
BMC ophthalmology
ISSN: 1471-2415
Titre abrégé: BMC Ophthalmol
Pays: England
ID NLM: 100967802
Informations de publication
Date de publication:
09 Oct 2024
09 Oct 2024
Historique:
received:
06
05
2024
accepted:
26
09
2024
medline:
10
10
2024
pubmed:
10
10
2024
entrez:
9
10
2024
Statut:
epublish
Résumé
We report two cases of fungal endophthalmitis induced by Candida species identified based on internal transcribed spacer 1 (ITS1) sequencing. In two cases, endophthalmitis was suspected, and the patients underwent pars plana vitrectomy. Case 1 was a 64-year-old woman with a history of cataract surgery 10 days prior. She had a history of anal primary melanoma, which metastasized throughout the body and subsequently relapsed. Vitreous culture and ITS-1 deep sequencing revealed the presence of the rare fungus, Candida dubliniensis. Case 2 was a 54-year-old man with a history of liver cancer and kidney failure. Culture methods and ITS1 deep sequencing both revealed the presence of Candida albicans. Both patients exhibited good visual prognoses after treatment with topical and systemic antibiotics. We present two cases of fungal endophthalmitis caused by two Candida species identified by both the culture method and ITS1 deep sequencing. The fungal pathogen was identified by ITS deep sequencing three days after sample submission; the culture method yielded results after 1 week. These findings support the applicability of ITS1 sequencing for timely pathogen identification for cases of fungal endophthalmitis and provide detailed taxonomic information at the species level.
Sections du résumé
BACKGROUND
BACKGROUND
We report two cases of fungal endophthalmitis induced by Candida species identified based on internal transcribed spacer 1 (ITS1) sequencing.
CASE PRESENTATION
METHODS
In two cases, endophthalmitis was suspected, and the patients underwent pars plana vitrectomy. Case 1 was a 64-year-old woman with a history of cataract surgery 10 days prior. She had a history of anal primary melanoma, which metastasized throughout the body and subsequently relapsed. Vitreous culture and ITS-1 deep sequencing revealed the presence of the rare fungus, Candida dubliniensis. Case 2 was a 54-year-old man with a history of liver cancer and kidney failure. Culture methods and ITS1 deep sequencing both revealed the presence of Candida albicans. Both patients exhibited good visual prognoses after treatment with topical and systemic antibiotics.
CONCLUSIONS
CONCLUSIONS
We present two cases of fungal endophthalmitis caused by two Candida species identified by both the culture method and ITS1 deep sequencing. The fungal pathogen was identified by ITS deep sequencing three days after sample submission; the culture method yielded results after 1 week. These findings support the applicability of ITS1 sequencing for timely pathogen identification for cases of fungal endophthalmitis and provide detailed taxonomic information at the species level.
Identifiants
pubmed: 39385149
doi: 10.1186/s12886-024-03702-4
pii: 10.1186/s12886-024-03702-4
doi:
Substances chimiques
DNA, Fungal
0
Antifungal Agents
0
Types de publication
Case Reports
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
444Subventions
Organisme : Japan Society for the Promotion of Science
ID : 23K159060
Organisme : Japan Society for the Promotion of Science
ID : 21K08505
Informations de copyright
© 2024. The Author(s).
Références
Durand ML. Bacterial and fungal endophthalmitis. Clin Microbiol Rev. 2017;30:597–613.
doi: 10.1128/CMR.00113-16
pubmed: 28356323
pmcid: 5475221
Kessel L, Flesner P, Andresen J, Erngaard D, Tendal B, Hjortdal J. Antibiotic prevention of postcataract endophthalmitis: a systematic review and meta-analysis. Acta Ophthalmol. 2015;93:303–17.
doi: 10.1111/aos.12684
pubmed: 25779209
pmcid: 6680152
Xu K, Chin EK, Bennett SR, Williams DF, Ryan EH, Dev S, et al. Endophthalmitis after intravitreal injection of vascular endothelial growth factor inhibitors: management and visual outcomes. Ophthalmology. 2018;125:1279–86.
doi: 10.1016/j.ophtha.2018.01.022
pubmed: 29477689
Vinekar A, Dogra MR, Avadhani K, Gupta V, Gupta A, Chakrabarti A. Management of recurrent postoperative fungal endophthalmitis. Indian J Ophthalmol. 2014;62:136–40.
doi: 10.4103/0301-4738.128588
pubmed: 24618484
pmcid: 4005226
Kim SW, Kim JH, Choi M, Lee SJ, Shin JP, Kim JG, et al. An outbreak of fungal endophthalmitis after cataract surgery in South Korea. JAMA Ophthalmol. 2023;141:226–33.
doi: 10.1001/jamaophthalmol.2022.5927
pubmed: 36656597
pmcid: 9857837
Haseeb AA, Elhusseiny AM, Siddiqui MZ, Ahmad KT, Sallam AB. Fungal endophthalmitis: a comprehensive review. J Fungi (Basel). 2021;7:996.
doi: 10.3390/jof7110996
pubmed: 34829283
Breazzano MP, Bond JB III, Bearelly S, Kim DH, Donahue SP, Lum F, et al. American Academy of Ophthalmology recommendations on screening for endogenous Candida Endophthalmitis. Ophthalmology. 2022;129:73–6.
doi: 10.1016/j.ophtha.2021.07.015
pubmed: 34293405
Ramírez-Soto MC, Bonifaz A. Ocular fungal infections. J Fungi (Basel). 2022;8:1078.
doi: 10.3390/jof8101078
pubmed: 36294643
Talapko J, Juzbašić M, Matijević T, Pustijanac E, Bekić S, Kotris I, et al. Candida albicans-the virulence factors and clinical manifestations of infection. J Fungi (Basel). 2021;7:79.
doi: 10.3390/jof7020079
pubmed: 33499276
Ueda T, Takesue Y, Tokimatsu I, Miyazaki T, Nakada-Motokawa N, Nagao M, et al. The incidence of endophthalmitis or macular involvement and the necessity of a routine ophthalmic examination in patients with candidemia. PLoS ONE. 2019;14:e0216956.
doi: 10.1371/journal.pone.0216956
pubmed: 31120928
pmcid: 6532890
Hillenbrand M, Mendy A, Patel K, Wilkinson R, Liao S, Robertson J, et al. The incidence of ocular complications in candidemic patients and implications for the practice of routine eye exams. Open Forum Infect Dis. 2022;9:ofac045.
doi: 10.1093/ofid/ofac045
pubmed: 35355893
pmcid: 8962754
Chen KJ, Sun MH, Chen YP, Chen YH, Wang NK, Liu L, et al. Endogenous fungal endophthalmitis: causative organisms, treatments, and visual outcomes. J Fungi (Basel). 2022;8:641.
doi: 10.3390/jof8060641
pubmed: 35736124
O’Donnell M, Eller AW, Waxman EL, Clancy CJ, Nguyen MH. Screening for ocular candidiasis among patients with candidemia: is it time to change practice? Clin Infect Dis. 2022;75:1092–6.
doi: 10.1093/cid/ciac233
pubmed: 35325089
Maione A, Bellavita R, de Alteriis E, Galdiero S, Albarano L, La Pietra A, et al. WMR peptide as antifungal and antibiofilm against albicans and non-albicans Candida species: shreds of evidence on the mechanism of action. Int J Mol Sci. 2022;23:2151.
doi: 10.3390/ijms23042151
pubmed: 35216270
pmcid: 8879636
Kishore K, McGowan DS, Chatterjee T, Hassanzadeh B. A case of bilateral endogenous Candida dubliniensis endophthalmitis treated with aggressive local and systemic therapy. Case Rep Ophthalmol. 2020;11:561–73.
doi: 10.1159/000510500
pubmed: 33250757
pmcid: 7670387
Banos S, Lentendu G, Kopf A, Wubet T, Glöckner FO, Reich M. A comprehensive fungi-specific 18S rRNA gene sequence primer toolkit suited for diverse research issues and sequencing platforms. BMC Microbiol. 2018;18:190.
doi: 10.1186/s12866-018-1331-4
pubmed: 30458701
pmcid: 6247509
Motooka D, Fujimoto K, Tanaka R, Yaguchi T, Gotoh K, Maeda Y, et al. Fungal ITS1 deep-sequencing strategies to reconstruct the composition of a 26-species community and evaluation of the gut mycobiota of healthy Japanese individuals. Front Microbiol. 2017;8:238.
doi: 10.3389/fmicb.2017.00238
pubmed: 28261190
pmcid: 5309391
Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA, et al. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proc Natl Acad Sci U S A. 2012;109:6241–6.
doi: 10.1073/pnas.1117018109
pubmed: 22454494
pmcid: 3341068
Asao K, Hashida N, Maruyama K, Motooka D, Tsukamoto T, Usui Y, et al. Comparative evaluation of 16S rRNA metagenomic sequencing in the diagnosis and understanding of bacterial endophthalmitis. BMJ Open Ophthalmol. 2023;8:e001342.
doi: 10.1136/bmjophth-2023-001342
pubmed: 37709670
pmcid: 10503327
Peng KL, Kung YH, Tsai HS, Wu TT. Treatment outcomes of acute postoperative infectious endophthalmitis. BMC Ophthalmol. 2021;21:384.
doi: 10.1186/s12886-021-02144-6
pubmed: 34715824
pmcid: 8555216
Khan Z, Ahmad S, Joseph L, Chandy R. Candida Dubliniensis: an appraisal of its clinical significance as a bloodstream pathogen. PLoS ONE. 2012;7:e32952.
doi: 10.1371/journal.pone.0032952
pubmed: 22396802
pmcid: 3292580
Mirhendi H, Makimura K, Zomorodian K, Maeda N, Ohshima T, Yamaguchi H. Differentiation of Candida albicans and Candida dubliniensis using a single-enzyme PCR-RFLP method. Jpn J Infect Dis. 2005;58:235–7.
doi: 10.7883/yoken.JJID.2005.235
pubmed: 16116258
Danielescu C, Stanca HT, Iorga RE, Darabus DM, Potop V. The diagnosis and treatment of fungal endophthalmitis: an update. Diagnostics (Basel). 2022;12:679.
doi: 10.3390/diagnostics12030679
pubmed: 35328231
Ayadi R, Sitterlé E, d’Enfert C, Dannaoui E, Bougnoux ME. Candida albicans and Candida Dubliniensis Show different trailing effect patterns when exposed to echinocandins and Azoles. Front Microbiol. 2020;11:1286.
doi: 10.3389/fmicb.2020.01286
pubmed: 32612593
pmcid: 7308431
Kianipour S, Ardestani ME, Dehghan P. Identification of Candida albicans and Candida Dubliniensis Species isolated from bronchoalveolar lavage samples using genotypic and phenotypic methods. Adv Biomed Res. 2018;7:66.
doi: 10.4103/abr.abr_138_17
pubmed: 29862215
pmcid: 5952538
Sullivan DJ, Moran GP, Coleman DC. Candida Dubliniensis: ten years on. FEMS Microbiol Lett. 2005;253:9–17.
doi: 10.1016/j.femsle.2005.09.015
pubmed: 16213674
Scorzoni L, de Paula E, Silva ACA, Marcos CM, Assato PA, de Melo WCMA, de Oliveira HC, et al. Antifungal therapy: new advances in the understanding and treatment of mycosis. Front Microbiol. 2017;8:36.
doi: 10.3389/fmicb.2017.00036
pubmed: 28167935
pmcid: 5253656
Vitale RG. Role of antifungal combinations in difficult to treat candida infections. J Fungi (Basel). 2021;7:34575770.
Barantsevich N, Barantsevich E. Diagnosis and treatment of invasive candidiasis. Antibiot (Basel). 2022;11:718.
doi: 10.3390/antibiotics11060718
Reginatto P, Agostinetto GJ, Fuentefria RDN, Marinho DR, Pizzol MD, Fuentefria AM. Eye fungal infections: a mini review. Arch Microbiol. 2023;205:236.
doi: 10.1007/s00203-023-03536-6
pubmed: 37183227
pmcid: 10183313
Lakhani P, Patil A, Majumdar S. Challenges in the polyene- and azole-based pharmacotherapy of ocular fungal infections. J Ocul Pharmacol Ther. 2019;35:6–22.
doi: 10.1089/jop.2018.0089
pubmed: 30481082
pmcid: 6354613
Chiu CY, Miller SA. Clinical metagenomics. Nat Rev Genet. 2019;20:341–55.
doi: 10.1038/s41576-019-0113-7
pubmed: 30918369
pmcid: 6858796
La Reau AJ, Strom NB, Filvaroff E, Mavrommatis K, Ward TL, Knights D. Shallow shotgun sequencing reduces technical variation in microbiome analysis. Sci Rep. 2023;13:7668.
doi: 10.1038/s41598-023-33489-1
pubmed: 37169816
pmcid: 10175443
Lema NK, Gemeda MT, Woldesemayat AA. Recent advances in metagenomic approaches, applications, and challenges. Curr Microbiol. 2023;80:347.
doi: 10.1007/s00284-023-03451-5
pubmed: 37733134
Batool M, Galloway-Peña J. Clinical metagenomics—challenges and future prospects. Front Microbiol. 2023;14: 1186424.2023:1186424.