Molecular epidemiological analysis of bla
Humans
Klebsiella pneumoniae
/ genetics
Whole Genome Sequencing
Klebsiella Infections
/ epidemiology
Disease Outbreaks
Child
beta-Lactamases
/ genetics
China
/ epidemiology
Virulence Factors
/ genetics
Plasmids
/ genetics
Biofilms
/ growth & development
Molecular Epidemiology
Anti-Bacterial Agents
/ pharmacology
Child, Preschool
Bacterial Proteins
/ genetics
Microbial Sensitivity Tests
Carbapenems
/ pharmacology
Female
Male
Genome, Bacterial
Infant
Carbapenem-Resistant Enterobacteriaceae
/ genetics
Klebsiella pneumoniae
bla
NDM−5
Biofilm
IncX3
Pediatric infection control
ST2407_K25
Journal
Annals of clinical microbiology and antimicrobials
ISSN: 1476-0711
Titre abrégé: Ann Clin Microbiol Antimicrob
Pays: England
ID NLM: 101152152
Informations de publication
Date de publication:
09 Oct 2024
09 Oct 2024
Historique:
received:
18
02
2023
accepted:
20
09
2024
medline:
10
10
2024
pubmed:
10
10
2024
entrez:
10
10
2024
Statut:
epublish
Résumé
Pediatric patients are vulnerable to the threat of carbapenem-resistant Klebsiella pneumoniae (CRKP) due to their limited immunity and few available antibiotics. Especially when these pathogens exhibit hypervirulent phenotypes, they are often associated with poor clinical outcomes. In this study, we investigated a CRKP outbreak in pediatric patients from 2019 to 2021 in a teaching hospital in China based on whole genome sequencing. We sequenced twenty-nine CRKP isolates isolated from unduplicated pediatric patients to understand their genetic relationships, virulence factors, resistance mechanisms, and transmission trajectories. Conjugation experiments were performed to evaluate the horizontal transfer ability of carbapenem resistance determinants in twenty-nine CRKP isolates. We then characterized these isolates for biofilm formation ability and serum resistance. Genetic relatedness, comparison of plasmids, and chromosomal locus variation of CRKP isolates were analyzed by bioinformatics. All the isolates were carbapenemase-producers harbouring bla Our study provides evidence of the clonal spread of bla
Sections du résumé
BACKGROUND
BACKGROUND
Pediatric patients are vulnerable to the threat of carbapenem-resistant Klebsiella pneumoniae (CRKP) due to their limited immunity and few available antibiotics. Especially when these pathogens exhibit hypervirulent phenotypes, they are often associated with poor clinical outcomes.
METHODS
METHODS
In this study, we investigated a CRKP outbreak in pediatric patients from 2019 to 2021 in a teaching hospital in China based on whole genome sequencing. We sequenced twenty-nine CRKP isolates isolated from unduplicated pediatric patients to understand their genetic relationships, virulence factors, resistance mechanisms, and transmission trajectories. Conjugation experiments were performed to evaluate the horizontal transfer ability of carbapenem resistance determinants in twenty-nine CRKP isolates. We then characterized these isolates for biofilm formation ability and serum resistance. Genetic relatedness, comparison of plasmids, and chromosomal locus variation of CRKP isolates were analyzed by bioinformatics.
RESULTS
RESULTS
All the isolates were carbapenemase-producers harbouring bla
CONCLUSIONS
CONCLUSIONS
Our study provides evidence of the clonal spread of bla
Identifiants
pubmed: 39385261
doi: 10.1186/s12941-024-00747-7
pii: 10.1186/s12941-024-00747-7
doi:
Substances chimiques
beta-Lactamases
EC 3.5.2.6
Virulence Factors
0
Anti-Bacterial Agents
0
Bacterial Proteins
0
Carbapenems
0
carbapenemase
EC 3.5.2.6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
91Subventions
Organisme : Department of Science and Technology of Sichuan Province,China
ID : 2021YFS0171
Organisme : Department of Science and Technology of Sichuan Province,China
ID : 2022YFS0008
Organisme : Southwest Medical University
ID : 2019ZQN017
Informations de copyright
© 2024. The Author(s).
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