TFIIS is required for reproductive development and thermal adaptation in barley.
Barley (Hordeum vulgare L.)
Heat stress
Reproductive development
TFIIS
Transcriptional elongation
Journal
Plant cell reports
ISSN: 1432-203X
Titre abrégé: Plant Cell Rep
Pays: Germany
ID NLM: 9880970
Informations de publication
Date de publication:
10 Oct 2024
10 Oct 2024
Historique:
received:
26
07
2024
accepted:
01
10
2024
medline:
11
10
2024
pubmed:
11
10
2024
entrez:
10
10
2024
Statut:
epublish
Résumé
Barley reproductive fitness and efficient heat stress adaptation requires the activity of TFIIS, the elongation cofactor of RNAPII. Regulation of transcriptional machinery and its adaptive role under different stress conditions are studied extensively in the dicot model plant Arabidopsis, but our knowledge on monocot species remains elusive. TFIIS is an RNA polymerase II-associated transcription elongation cofactor. Previously, it was shown that TFIIS ensures efficient transcription elongation that is necessary for heat stress survival in A. thaliana. However, the function of TFIIS has not been analysed in monocots. In the present work, we have generated and studied independent tfIIs-crispr-mutant barley lines. We show that TFIIS is needed for reproductive development and heat stress survival in barley. The molecular basis of HS-sensitivity of tfIIs mutants is the retarded expression of heat stress protein transcripts, which leads to late accumulation of HSP chaperones, enhanced proteotoxicity and ultimately to lethality. We also show that TFIIS is transcriptionally regulated in response to heat, supporting a conserved adaptive function of these control elements for plant thermal adaptation. In sum, our results are a step forward for the better understanding of transcriptional machinery regulation in monocot crops.
Identifiants
pubmed: 39390135
doi: 10.1007/s00299-024-03345-1
pii: 10.1007/s00299-024-03345-1
doi:
Substances chimiques
Plant Proteins
0
transcription factor S-II
0
Transcriptional Elongation Factors
0
Heat-Shock Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
260Subventions
Organisme : Hungarian Scientific Research Fund
ID : K-137722
Organisme : Hungarian Scientific Research Fund
ID : K-146300
Organisme : Hungarian Scientific Research Fund
ID : K-139349
Organisme : Hungarian Scientific Research Fund
ID : K-134914
Organisme : Magyar Agrár- és Élettudományi Egyetem
ID : Flagship Research group Programme
Organisme : HORIZON EUROPE Framework Programme
ID : EU Horizon 2020 grant No. 739593
Organisme : NKFIH
ID : KIM NKFIA 2022-2.1.1-NL-2022-00005
Organisme : Magyar Tudományos Akadémia
ID : BO/00015/21
Informations de copyright
© 2024. The Author(s).
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