Changes in the proteomics and metabolomics profiles of Cormus Domestica (L.) fruits during the ripening process.
Cormus Domestica L.
Ethnobotanical heritage
Metabolomics
Post-harvest
Proteomics
Journal
BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807
Informations de publication
Date de publication:
10 Oct 2024
10 Oct 2024
Historique:
received:
20
12
2023
accepted:
07
10
2024
medline:
11
10
2024
pubmed:
11
10
2024
entrez:
10
10
2024
Statut:
epublish
Résumé
Cormus domestica (L.) is a monophyletic wild fruit tree belonging to the Rosaceae family, with well-documented use in the Mediterranean region. Traditionally, these fruits are harvested and stored for at least 2 weeks before consumption. During this period, the fruit reaches its well-known and peculiar organoleptic and texture characteristics. However, the spread of more profitable fruit tree species, resulted in its progressive erosion. In this work we performed proteomic and metabolomic fruit analyses at three times after harvesting, to characterise postharvest physiological and molecular changes, it related to nutritional and organoleptic properties at consumption. Proteomics and metabolomics analysis were performed on fruits harvested at different time points: freshly harvested fruit (T0), fruit two weeks after harvest (T1) and fruit four weeks after harvest (T2). Proteomic analysis (Shotgun Proteomic in LC-MS/MS) resulted in 643 proteins identified. Most of the differentially abundant proteins between the three phases observed were involved in the softening process, carbohydrate metabolism and stress responses. Enzymes, such as xyloglucan endotransglucosylase/hydrolase, pectin acetylesterase, beta-galactosidase and pectinesterase, accumulated during fruit ripening and could explain the pulp breakdown observed in C. domestica. At the same time, enzymes abundant in the early stages (T0), such as sucrose synthase and malic enzyme, explain the accumulation of sugars and the lowering of acidity during the process. The metabolites extraction from C. domestica fruits enabled the identification of 606 statistically significant differentially abundant metabolites. Some compounds such as piptamine and resorcinol, well-known for their antimicrobial and antifungal properties, and several bioactive compounds such as endocannabinoids, usually described in the leaves, accumulate in C. domestica fruit during the post-harvest process. The metabolomic and proteomic profiling of the C. domestica fruit during the postharvest process, evaluated in the study, provides a considerable contribution to filling the existing information gap, enabling the molecular and phytochemical characterisation of this erosion-endangered fruit. Data show biochemical changes that transform the harvested fruit into palatable consumable product.
Sections du résumé
BACKGROUND
BACKGROUND
Cormus domestica (L.) is a monophyletic wild fruit tree belonging to the Rosaceae family, with well-documented use in the Mediterranean region. Traditionally, these fruits are harvested and stored for at least 2 weeks before consumption. During this period, the fruit reaches its well-known and peculiar organoleptic and texture characteristics. However, the spread of more profitable fruit tree species, resulted in its progressive erosion. In this work we performed proteomic and metabolomic fruit analyses at three times after harvesting, to characterise postharvest physiological and molecular changes, it related to nutritional and organoleptic properties at consumption.
RESULTS
RESULTS
Proteomics and metabolomics analysis were performed on fruits harvested at different time points: freshly harvested fruit (T0), fruit two weeks after harvest (T1) and fruit four weeks after harvest (T2). Proteomic analysis (Shotgun Proteomic in LC-MS/MS) resulted in 643 proteins identified. Most of the differentially abundant proteins between the three phases observed were involved in the softening process, carbohydrate metabolism and stress responses. Enzymes, such as xyloglucan endotransglucosylase/hydrolase, pectin acetylesterase, beta-galactosidase and pectinesterase, accumulated during fruit ripening and could explain the pulp breakdown observed in C. domestica. At the same time, enzymes abundant in the early stages (T0), such as sucrose synthase and malic enzyme, explain the accumulation of sugars and the lowering of acidity during the process. The metabolites extraction from C. domestica fruits enabled the identification of 606 statistically significant differentially abundant metabolites. Some compounds such as piptamine and resorcinol, well-known for their antimicrobial and antifungal properties, and several bioactive compounds such as endocannabinoids, usually described in the leaves, accumulate in C. domestica fruit during the post-harvest process.
CONCLUSIONS
CONCLUSIONS
The metabolomic and proteomic profiling of the C. domestica fruit during the postharvest process, evaluated in the study, provides a considerable contribution to filling the existing information gap, enabling the molecular and phytochemical characterisation of this erosion-endangered fruit. Data show biochemical changes that transform the harvested fruit into palatable consumable product.
Identifiants
pubmed: 39390371
doi: 10.1186/s12870-024-05677-2
pii: 10.1186/s12870-024-05677-2
doi:
Substances chimiques
Plant Proteins
0
Proteome
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
945Subventions
Organisme : Regione Campania
ID : PSR Campania 2014/2020 Misura 10 - Tipologia di Intervento 10.2.1-Conservazione delle risorse genetiche autoctone a tutela della biodiversità - RISORSE GENETICHE VEGETALI PROGETTO "DICOVALE: Diversità, conservazione e valorizzazione delle specie legnose da frutto autoctone campane." - CUP n° B24I19000470009
Organisme : Regione Campania
ID : PSR Campania 2014/2020 Misura 10 - Tipologia di Intervento 10.2.1-Conservazione delle risorse genetiche autoctone a tutela della biodiversità - RISORSE GENETICHE VEGETALI PROGETTO "DICOVALE: Diversità, conservazione e valorizzazione delle specie legnose da frutto autoctone campane." - CUP n° B24I19000470009
Organisme : Regione Campania
ID : PSR Campania 2014/2020 Misura 10 - Tipologia di Intervento 10.2.1-Conservazione delle risorse genetiche autoctone a tutela della biodiversità - RISORSE GENETICHE VEGETALI PROGETTO "DICOVALE: Diversità, conservazione e valorizzazione delle specie legnose da frutto autoctone campane." - CUP n° B24I19000470009
Organisme : Regione Campania
ID : PSR Campania 2014/2020 Misura 10 - Tipologia di Intervento 10.2.1-Conservazione delle risorse genetiche autoctone a tutela della biodiversità - RISORSE GENETICHE VEGETALI PROGETTO "DICOVALE: Diversità, conservazione e valorizzazione delle specie legnose da frutto autoctone campane." - CUP n° B24I19000470009
Organisme : Regione Campania
ID : PSR Campania 2014/2020 Misura 10 - Tipologia di Intervento 10.2.1-Conservazione delle risorse genetiche autoctone a tutela della biodiversità - RISORSE GENETICHE VEGETALI PROGETTO "DICOVALE: Diversità, conservazione e valorizzazione delle specie legnose da frutto autoctone campane." - CUP n° B24I19000470009
Organisme : Regione Campania
ID : PSR Campania 2014/2020 Misura 10 - Tipologia di Intervento 10.2.1-Conservazione delle risorse genetiche autoctone a tutela della biodiversità - RISORSE GENETICHE VEGETALI PROGETTO "DICOVALE: Diversità, conservazione e valorizzazione delle specie legnose da frutto autoctone campane." - CUP n° B24I19000470009
Organisme : Regione Campania
ID : PSR Campania 2014/2020 Misura 10 - Tipologia di Intervento 10.2.1-Conservazione delle risorse genetiche autoctone a tutela della biodiversità - RISORSE GENETICHE VEGETALI PROGETTO "DICOVALE: Diversità, conservazione e valorizzazione delle specie legnose da frutto autoctone campane." - CUP n° B24I19000470009
Organisme : Regione Campania
ID : PSR Campania 2014/2020 Misura 10 - Tipologia di Intervento 10.2.1-Conservazione delle risorse genetiche autoctone a tutela della biodiversità - RISORSE GENETICHE VEGETALI PROGETTO "DICOVALE: Diversità, conservazione e valorizzazione delle specie legnose da frutto autoctone campane." - CUP n° B24I19000470009
Organisme : Regione Campania
ID : PSR Campania 2014/2020 Misura 10 - Tipologia di Intervento 10.2.1-Conservazione delle risorse genetiche autoctone a tutela della biodiversità - RISORSE GENETICHE VEGETALI PROGETTO "DICOVALE: Diversità, conservazione e valorizzazione delle specie legnose da frutto autoctone campane." - CUP n° B24I19000470009
Informations de copyright
© 2024. The Author(s).
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