A protocol to isolate and characterize pure monocytes and generate monocyte-derived dendritic cells through FBS-Coated flasks.
Humans
Dendritic Cells
/ cytology
Monocytes
/ cytology
Granulocyte-Macrophage Colony-Stimulating Factor
/ metabolism
Interleukin-4
/ metabolism
Cell Differentiation
Cell Separation
/ methods
Flow Cytometry
/ methods
HLA-DR Antigens
/ metabolism
CD83 Antigen
Cells, Cultured
Cell Culture Techniques
/ methods
Antigens, CD
/ metabolism
Monocyte
Monocytes-derived dendritic cells
hrGM-CSF
hrIL-4
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
14 Oct 2024
14 Oct 2024
Historique:
received:
21
02
2024
accepted:
04
10
2024
medline:
14
10
2024
pubmed:
14
10
2024
entrez:
13
10
2024
Statut:
epublish
Résumé
This study explores methods to isolate high-pure monocytes and optimize the best growth factor concentration to generate monocytes-derived dendritic cells (mo-DCs), subset DC1, which is crucial in immune responses. Three protocols for monocyte isolation from peripheral blood mononuclear cells (PBMCs) were evaluated: three-hour incubation on FBS-coated flasks; an overnight incubation on FBS-coated flasks; and Magnetic Activated Cell Sorting (MACS). Additionally, five different concentrations of human recombinant granulocyte-macrophage colony-stimulating factor (hrGM-CSF) and human recombinant interleukin-4 (hrIL-4) were compared. We used Flow cytometry to assess the isolation, purification, and generation of pure monocytes characterized as CD14
Identifiants
pubmed: 39397067
doi: 10.1038/s41598-024-75376-3
pii: 10.1038/s41598-024-75376-3
doi:
Substances chimiques
Granulocyte-Macrophage Colony-Stimulating Factor
83869-56-1
Interleukin-4
207137-56-2
HLA-DR Antigens
0
CD83 Antigen
0
Antigens, CD
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
23956Informations de copyright
© 2024. The Author(s).
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