Manipulating the nucleolar serine-rich protein Srp40p in Saccharomyces cerevisiae may improve isobutanol production.
SRP40
Saccharomyces cerevisiae
Isobutanol
Transcriptome analysis
Journal
World journal of microbiology & biotechnology
ISSN: 1573-0972
Titre abrégé: World J Microbiol Biotechnol
Pays: Germany
ID NLM: 9012472
Informations de publication
Date de publication:
15 Oct 2024
15 Oct 2024
Historique:
received:
15
05
2024
accepted:
20
09
2024
medline:
15
10
2024
pubmed:
15
10
2024
entrez:
15
10
2024
Statut:
epublish
Résumé
Isobutanol represents a promising second-generation biofuel. Saccharomyces cerevisiae can produce minor quantities of isobutanol as a byproduct. Increasing yeast tolerance to isobutanol is a crucial step toward achieving higher production levels. Previously, we discovered that expression of the srp40 gene could increase S. cerevisiae isobutanol tolerance. In this study, we explored the impact of overexpressing srp40 on isobutanol production. We used the CEN/ARS plasmid YCplac22-srp40 to overexpress srp40 in S. cerevisiae strain W303-1A. The resulting strain was named W303-1A-srp40. We subsequently performed metabolic engineering of isobutanol synthesis by overexpressing ILV2, ILV3 and ARO10 in W303-1 A-srp40. The resulting strain was named 303V2V3A10-22-srp40. Our findings revealed that, compared with the control strain, the 303V2V3A10-22-srp40 strain amplified isobutanol production by 50%. A transcriptome analysis revealed that upregulated genes associated with aminoacyl-tRNA biosynthesis or downregulated genes associated with phenylalanine, tyrosine, and tryptophan biosynthesis might yield increased isobutanol production in 303V2V3A10-22-srp40. Moreover, the decreases in the biosynthesis of amino acids and oxidative phosphorylation might play pivotal roles in the increased isobutanol tolerance of strain W303-1A-srp40. In summary, the overexpression of srp40 could increase isobutanol production and tolerance in S. cerevisiae. This study offers novel insights regarding strategies for increasing isobutanol production.
Identifiants
pubmed: 39404979
doi: 10.1007/s11274-024-04150-4
pii: 10.1007/s11274-024-04150-4
doi:
Substances chimiques
isobutyl alcohol
56F9Z98TEM
Butanols
0
Saccharomyces cerevisiae Proteins
0
Biofuels
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
349Subventions
Organisme : the National Natural Science Foundation of China
ID : NSFC-21206028, NSFC-21978065
Organisme : Chunhui Project Foundation of the Education Department of China
ID : No. Z2017012
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature B.V.
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