Blood-rsCDM: a new rapid and simplified carbapenemase detection method for detecting carbapenemases in Enterobacterales directly from positive blood cultures.
beta-Lactamases
/ genetics
Bacterial Proteins
/ genetics
Humans
Blood Culture
/ methods
Sensitivity and Specificity
Enterobacteriaceae
/ enzymology
Boronic Acids
/ pharmacology
Edetic Acid
/ pharmacology
Enterobacteriaceae Infections
/ microbiology
beta-Lactamase Inhibitors
/ pharmacology
Carbapenems
/ pharmacology
Microbial Sensitivity Tests
Anti-Bacterial Agents
/ pharmacology
Blood-rsCDM
Carbapenemase
Carbapenemase-producing Enterobacterales
Phenotypic assay
Journal
BMC microbiology
ISSN: 1471-2180
Titre abrégé: BMC Microbiol
Pays: England
ID NLM: 100966981
Informations de publication
Date de publication:
16 Oct 2024
16 Oct 2024
Historique:
received:
31
08
2023
accepted:
26
09
2024
medline:
17
10
2024
pubmed:
17
10
2024
entrez:
16
10
2024
Statut:
epublish
Résumé
We aim to validate and evaluate a new rapid and simplified method, called Blood-rsCDM, for the detection and characterization of carbapenemase using 3-aminophenylboronic acid (APBA) and ethylenediaminetetraacetic acid (EDTA) β-lactamase inhibitors from positive blood cultures. We utilized a panel of 172 Enterobacterales strains, including blaKPC (77), blaNDM (48), blaIMP (9), blaVIM (2), blaOXA-181 (2), blaKPC and blaNDM (6), as well as 28 carbapenem-susceptible Enterobacterales isolates, to assess the performance of Blood-rsCDM and the EDTA-carbapenem inactivation method (eCIM). Carbapenemase class was determined using specific inhibitors at 4 h and 6 h by Blood-rsCDM. Blood-rsCDM exhibited a sensitivity of 97.9% at both time points, with a specificity of 100%, regardless of the culture duration. The sensitivity of eCIM was 94.4%, with a specificity of 100%. Blood-rsCDM accurately characterized KPC-producing isolates as 77/77, metallo-β-lactamases (MBLs) as 58/59, and KPC and NDM carbapenemases as 6/6 at 4 h. There was no difference in results between the 4 h and 6 h time points. However, Blood-rsCDM could not differentiate OXA-181-producing strains. For eCIM, the characterization numbers for KPC-, OXA-181-, and MBLs-producing strains were 77/77, 2/2, and 57/59, respectively, but it failed to detect the coproduction of KPC and NDM isolates. Blood-rsCDM accurately discriminates carbapenemase within 4 h and is capable of directly differentiating multi-enzyme (KPC and NDM) presence from positive blood culture broths. Therefore, Blood-rsCDM represents a rapid, simple, easy-to-read, and accurate tool that can be utilized in resource-limited settings.
Identifiants
pubmed: 39415086
doi: 10.1186/s12866-024-03553-5
pii: 10.1186/s12866-024-03553-5
doi:
Substances chimiques
beta-Lactamases
EC 3.5.2.6
carbapenemase
EC 3.5.2.6
Bacterial Proteins
0
Boronic Acids
0
Edetic Acid
9G34HU7RV0
3-aminobenzeneboronic acid
30418-59-8
beta-Lactamase Inhibitors
0
Carbapenems
0
Anti-Bacterial Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
410Informations de copyright
© 2024. The Author(s).
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