Phytochrome interacting factor 3 mediates low light signaling to regulate isorhynchophylline biosynthesis in Uncaria rhynchophylla.
PIFs
Uncaria rhynchophylla
Isorhynchophylline
Low light
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
23 10 2024
23 10 2024
Historique:
received:
01
07
2024
accepted:
17
10
2024
medline:
24
10
2024
pubmed:
24
10
2024
entrez:
24
10
2024
Statut:
epublish
Résumé
Phytochrome interacting factors (PIFs) serve as crucial regulators in the light signal transduction pathway and also mediate light signals to regulate secondary metabolite synthesis in plants. However, the regulator role of PIFs in secondary metabolites often varies among different plants. Isorhynchophylline (IRN), an iconic secondary metabolite of Uncaria rhynchophylla, holds significant medicinal value. Low light induces the synthesis of IRN in previous research, but PIFs in U. rhynchophylla have not been studied to date. Building on this, we identified a PIF protein, UrPIF3, which possesses the typical conserved domains of the PIFs and is localized in the nucleus. Moreover, the expression level of UrPIF3 is consistently positively correlated with the expression of two key enzyme genes (UrSGD and UrSTR) in the IRN biosynthesis pathway, regardless of whether under low light or restoring light conditions. Yeast one-hybrid and dual-luciferase assays further demonstrated that UrPIF3 can directly upregulate UrSGD. Conversely, silencing UrPIF3 inhibits IRN synthesis, and significantly reduces the expression levels of UrSGD and UrSTR. In summary, our results suggest that under low light conditions, UrPIF3 can directly upregulate UrSGD and indirectly upregulate UrSTR, thereby promoting the synthesis of IRN.
Identifiants
pubmed: 39443584
doi: 10.1038/s41598-024-76939-0
pii: 10.1038/s41598-024-76939-0
doi:
Substances chimiques
Oxindoles
0
Plant Proteins
0
rhyncophylline
46BQ79VJ8D
Indole Alkaloids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
25032Subventions
Organisme : the Scientific Research Project of Ordinary Undergraduate Colleges and the Universities of Guizhou Provincial Department of Education
ID : Qian Jiao Ji [2022]No. 145
Organisme : the Guizhou Provincial Science and Technology Projects
ID : Qian Ke He Ji Chu-ZK[2022] General 502
Organisme : the Guizhou Provincial Science and Technology Projects
ID : Qian Ke He Ji Chu-ZK[2022] General096
Organisme : the Guizhou Minzu University (Youth) Fund Research Project
ID : GZMUZK[2023]QN06
Organisme : the Guizhou Minzu University Doctoral Research Initiation Project
ID : GZMUZK[2024]QD51
Organisme : the National Natural Science Foundation of China
ID : No. 32460146
Informations de copyright
© 2024. The Author(s).
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