Running in mice increases the expression of brain hemoglobin-related genes interacting with the GH/IGF-1 system.
Animals
Insulin-Like Growth Factor I
/ metabolism
Mice
Male
Brain
/ metabolism
Growth Hormone
/ metabolism
Physical Conditioning, Animal
Hemoglobins
/ metabolism
Running
/ physiology
Gene Expression Regulation
Insulin
/ metabolism
Blood Glucose
/ metabolism
Mice, Inbred C57BL
Hippocampus
/ metabolism
Brain
Exercise
Insulin resistance
Insulin-like growth factor-1
Rodent
Sedentary
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
26 Oct 2024
26 Oct 2024
Historique:
received:
29
08
2024
accepted:
22
10
2024
medline:
27
10
2024
pubmed:
27
10
2024
entrez:
27
10
2024
Statut:
epublish
Résumé
The beneficial effects of exercise are partly mediated via local or systemic functions of the insulin-like growth factor-1 (IGF-1) system. As IGF-1 increases local brain hemoglobin beta (Hbb) transcripts, we hypothesized that exercise could have similar effects. Mice were single-housed with free access to running wheels for seven days. After sacrifice and saline perfusion, the expression of 13 genes was quantified using real-time quantitative polymerase chain reaction (RT-qPCR) in three brain regions: the prefrontal cortex, motor cortex, and hippocampus. In addition, plasma insulin, glucose, homeostatic model assessment of IR (HOMA-IR), C-peptide, and IGF-1 were investigated. We show that hemoglobin-related transcripts (Hbb and 5'-aminolevulinate synthase 2 [Alas2]) increased 46-63% in the running group, while IGF-1-related genes [Igf1 / growth hormone receptor (Ghr)] decreased slightly (7%). There were also moderate to large correlations between Hbb- and IGF-1-related genes in the running group but not in the sedentary group. HOMA-IR, plasma glucose, and insulin changed marginally and non-significantly, but there was a trend toward an increase in plasma-IGF-1 in the running group. In conclusion, seven days of running increased Hbb-related transcripts in three brain regions. Hbb-related transcripts correlated with components of the brain IGF-1 system only in the running group.
Identifiants
pubmed: 39462081
doi: 10.1038/s41598-024-77489-1
pii: 10.1038/s41598-024-77489-1
doi:
Substances chimiques
Insulin-Like Growth Factor I
67763-96-6
Growth Hormone
9002-72-6
Hemoglobins
0
Insulin
0
Blood Glucose
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
25464Informations de copyright
© 2024. The Author(s).
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