Characterization of Staphylococcus aureus isolated from milk samples for their virulence, biofilm, and antimicrobial resistance.
Biofilms
/ drug effects
Milk
/ microbiology
Animals
Staphylococcus aureus
/ genetics
Anti-Bacterial Agents
/ pharmacology
Virulence
/ genetics
Microbial Sensitivity Tests
Methicillin-Resistant Staphylococcus aureus
/ genetics
Drug Resistance, Bacterial
/ genetics
Staphylococcal Infections
/ microbiology
Enterotoxins
/ genetics
Food Microbiology
Cattle
Spa typing
Staphylococcus aureus
Antimicrobial resistance (AMR)
Biofilm
Coagulase gene
Enterotoxin
High Resolution Melting (HRM)
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
27 10 2024
27 10 2024
Historique:
received:
03
11
2023
accepted:
01
10
2024
medline:
28
10
2024
pubmed:
28
10
2024
entrez:
28
10
2024
Statut:
epublish
Résumé
The Staphylococcus aureus (S. aureus) one of the important food borne pathogen from milk, which was investigated in this study. The isolates were screened for antimicrobial resistance, enterotoxin genes, biofilm formation, spa typing, coagulase gene polymorphism and accessory gene regulator types. The prevalence of S. aureus in milk samples was 34.4% (89/259). Methicillin resistant S. aureus (MRSA) was found at 27% (24/89) of the isolates, were classified as community acquired based on SCCmec typing. The 24.71% (22/89) isolates demonstrated multiple antimicrobial resistance (MAR) pattern. However, none of the isolates carried vancomycin and mupirocin resistance genes. The isolates were positive for sea and sed enterotoxin genes and exhibited high frequency of biofilm formation. The High-Resolution Melting and conventional spa typing revealed that the isolates had both animal and community-associated S. aureus clustered origins. Coagulase gene polymorphism and agr typing demonstrated variable genotypic patterns. The finding of this study establishes the prevalence of community associated, enterotoxigenic, biofilm forming and antimicrobial resistance among S. aureus from milk in Chennai city. This emphasizing a potential threat to public health which needs a continuous monitoring system and strategies to mitigate their spread across the food chain and achieve food safety.
Identifiants
pubmed: 39465266
doi: 10.1038/s41598-024-75076-y
pii: 10.1038/s41598-024-75076-y
doi:
Substances chimiques
Anti-Bacterial Agents
0
Enterotoxins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
25635Informations de copyright
© 2024. The Author(s).
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