Suppression of Pcdh8/paraxial protocadherin is required for efficient neighbor exchange in morphogenetic cell movement during zebrafish notochord formation.
Convergence and extension (C&E)
Neighbor exchange (NE)
Notochord
Paraxial protocadherin (PAPC)
Supracellular stresses
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
28 Oct 2024
28 Oct 2024
Historique:
received:
11
04
2024
accepted:
16
10
2024
medline:
28
10
2024
pubmed:
28
10
2024
entrez:
28
10
2024
Statut:
epublish
Résumé
In certain forms of collective cell migration, changes in neighboring cells (neighbor exchange, NE) are essential. In the axial mesoderm in zebrafish, for example, the notochord is established through cell movements known as convergence and extension (C&E), which involves NE. For NE to occur efficiently, the balance between cell-scale and supracellular stresses plays a crucial role, but the molecular basis of how these stresses are controlled remains unclear. In this study, we focused on Pcdh8/Paraxial protocadherin (PAPC), which is specifically suppressed in the region (notochord) where and at the time (early gastrula) when extensive C&E occurs. Forced expression of PAPCΔC (PAPC lacking its intracellular domain) persisted in the developing notochord and resulted in morphogenetic defects in zebrafish. PAPCΔC was found to downregulate NE in the notochord in a homophilic contact-dependent manner. By examining oil droplets inserted between cells, we revealed that while cell-scale stresses were apparently unaffected, the direction of bias in the supracellular stresses was stabilized by the introduction of PAPCΔC in the notochordal region. Taken together, our results suggest that suppression of PAPC in the notochord is required to modify supracellular stresses and provide the conditions in which NE occurs efficiently, thus promoting morphogenetic cell movements.
Identifiants
pubmed: 39465278
doi: 10.1038/s41598-024-76762-7
pii: 10.1038/s41598-024-76762-7
doi:
Substances chimiques
Cadherins
0
Zebrafish Proteins
0
Protocadherins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
25697Subventions
Organisme : Japan Society for the Promotion of Science
ID : JP22K06815
Organisme : Japan Society for the Promotion of Science
ID : JP22K11498
Organisme : Japan Agency for Medical Research and Development
ID : JP21wm0525026
Informations de copyright
© 2024. The Author(s).
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