RACK1 contributes to the upregulation of embryonic genes in a model of cardiac hypertrophy.
Blebbistatin
Cardiac hypertrophy
GATA4
Growth factors
Heart
NFAT2
Phenylephrine
RACK1
WT1
Zebrafish
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
28 Oct 2024
28 Oct 2024
Historique:
received:
30
07
2024
accepted:
10
10
2024
medline:
28
10
2024
pubmed:
28
10
2024
entrez:
28
10
2024
Statut:
epublish
Résumé
Receptors for activated C kinases (RACKs) have been shown to coordinate PKC-mediated hypertrophic signalling in mice. However, little information is available on its participation in embryonic gene expression. This study investigated the involvement of RACK1 in the expression of embryonic genes in a zebrafish (ZF) ex vivo heart culture model by using phenylephrine (PE) or a growth factors cocktail (GFs) as a prohypertrophic/regeneration stimulus. Blebbistatin (BL) inhibition has also been studied for its ability to block the signal transduction actions of some PEs. qRT‒PCR and immunoblot analyses confirmed the upregulation of RACK1 in the PE- and GFs-treated groups. BL administration counteracted PE-induced hypertrophy and downregulated RACK1 expression. Immunohistochemical analyses of the heart revealed the colocalization of RACK1 and embryonic genes, namely, Gata4, Wt1, and Nfat2, under stimulation, whereas these genes were expressed at lower levels in the BL treatment group. Culturing ZF heart cells activated via GFs treatment increased the expression of RACK1. The overexpression of RACK1 induced by the transfection of recombinant RACK1 cDNA in ZF heart cells increased the expression of embryonic genes, especially after one week of GFs treatment. In summary, these results support the involvement of RACK1 in the induction of embryonic genes during cardiac hypertrophy/GFs stimulation in a fish heart model, which can be used as an alternative study model for mammals.
Identifiants
pubmed: 39465301
doi: 10.1038/s41598-024-76138-x
pii: 10.1038/s41598-024-76138-x
doi:
Substances chimiques
Receptors for Activated C Kinase
0
Phenylephrine
1WS297W6MV
Zebrafish Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
25698Subventions
Organisme : Ministero dell'Università e della Ricerca
ID : PRIN2017FJSM9S_003
Informations de copyright
© 2024. The Author(s).
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