Molecular mechanism of flagellar motor rotation arrest in bacterial zoospores of Actinoplanes missouriensis before germination.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
29 Oct 2024
29 Oct 2024
Historique:
received:
06
06
2024
accepted:
18
10
2024
medline:
30
10
2024
pubmed:
30
10
2024
entrez:
30
10
2024
Statut:
epublish
Résumé
Zoospores of the filamentous actinomycete Actinoplanes missouriensis swim vigorously using flagella and stop swimming to initiate germination in response to nutrient exposure. However, the molecular mechanisms underlying swimming cessation remain unknown. A protein (FtgA) of unknown function encoded by a chemotaxis gene cluster (che cluster-1) was found to be required for flagellar rotation arrest; the zoospores of ftgA-knockout mutants kept swimming awkwardly after germination. An ftgA-overexpressing strain exhibited a non-flagellated phenotype. Isolation of a suppressor strain from this strain and further in vivo experiments revealed that the extended N-terminal region of FliN, a component of the C-ring of the flagellar basal body, was involved in the function of FtgA; FliN-P101S canceled the flagellar rotation arrest by FtgA, as well as the negative effect of ftgA-overexpression on flagellation. Furthermore, bacterial two-hybrid assays suggested that FtgA interacted not only with the C-terminal core region of FliN but also with chemotaxis regulatory proteins CheA1 and CheW1-2, which are encoded by che cluster-1. We propose the following working model of motility regulation in A. missouriensis zoospores: the chemotaxis sensory complex initially captures FtgA to allow zoospores to swim and then releases FtgA to stop flagellar rotation (i.e., swimming) in response to external nutrient signals.
Identifiants
pubmed: 39472762
doi: 10.1038/s42003-024-07104-6
pii: 10.1038/s42003-024-07104-6
doi:
Substances chimiques
Bacterial Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1405Subventions
Organisme : Ministry of Education, Culture, Sports, Science and Technology (MEXT)
ID : JP26252010
Organisme : Ministry of Education, Culture, Sports, Science and Technology (MEXT)
ID : JP18H02122
Organisme : Ministry of Education, Culture, Sports, Science and Technology (MEXT)
ID : JP17K07711
Organisme : Ministry of Education, Culture, Sports, Science and Technology (MEXT)
ID : JP20K05781
Organisme : Ministry of Education, Culture, Sports, Science and Technology (MEXT)
ID : JP19H05685
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : A3 Foresight Program
Informations de copyright
© 2024. The Author(s).
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