Integration of bioinformatics and cellular experiments unveils the role of SYT12 in gastric cancer.
Humans
Stomach Neoplasms
/ genetics
Computational Biology
/ methods
Prognosis
Synaptotagmins
/ genetics
Epithelial-Mesenchymal Transition
/ genetics
Female
Male
DNA Methylation
Gene Expression Regulation, Neoplastic
Cell Proliferation
/ genetics
Biomarkers, Tumor
/ genetics
Middle Aged
Cell Line, Tumor
Promoter Regions, Genetic
Adenocarcinoma
/ genetics
Cell Movement
/ genetics
Aged
Bioinformatics
EMT
Gastric cancer
Migration
Proliferation
SYT12
Journal
BMC cancer
ISSN: 1471-2407
Titre abrégé: BMC Cancer
Pays: England
ID NLM: 100967800
Informations de publication
Date de publication:
29 Oct 2024
29 Oct 2024
Historique:
received:
09
07
2024
accepted:
18
10
2024
medline:
30
10
2024
pubmed:
30
10
2024
entrez:
30
10
2024
Statut:
epublish
Résumé
This study employs integrated bioinformatics analysis and in vitro cellular experiments to elucidate the role of Synaptotagmin-12 (SYT12) in the progression of gastric cancer. We utilized databases and platforms such as Xiantao Academic Tools, UALCAN, Kaplan-Meier plotter analysis, and The Cancer Genome Atlas (TCGA) to extract datasets on SYT12 in gastric cancer. We analyzed the relationship between SYT12 expression and the clinicopathological features, prognosis, diagnosis, and immune infiltration of stomach adenocarcinoma (STAD) patients. Verification was conducted using samples from 31 gastric cancer patients. Additionally, in vitro cellular experiments were performed to determine the role and potential mechanisms of SYT12 in the malignant behavior of gastric cancer cells. Comprehensive bioinformatics analysis indicated that SYT12 is highly expressed in most cancers and is associated with promoter DeoxyriboNucleic Acid (DNA) methylation levels. SYT12 expression correlated with clinicopathological features, immune cell infiltration, immune checkpoint gene expression, and poor prognosis in STAD patients. In vitro experiments suggest that SYT12 may promote the proliferation and migration of gastric cancer cells by inducing epithelial-mesenchymal transition (EMT). This study highlights the significant role of SYT12 in gastric cancer, suggesting its potential as a new target for early diagnosis, treatment, immunological, and prognostic evaluation in gastric cancer, offering new insights for precision medicine in this disease.
Identifiants
pubmed: 39472897
doi: 10.1186/s12885-024-13077-w
pii: 10.1186/s12885-024-13077-w
doi:
Substances chimiques
Synaptotagmins
134193-27-4
Biomarkers, Tumor
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1331Subventions
Organisme : the Science and Technology Planning Project of Gansu Province
ID : 21CX6FD163
Organisme : the Second Group of Longyuan Young Talents in Gansu Province
ID : 2023-11
Informations de copyright
© 2024. The Author(s).
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