Interleukin-4 and its receptor alpha in paediatric uncomplicated malaria patients from a Ghanaian case-control study.
Humans
Interleukin-4
/ genetics
Ghana
/ epidemiology
Case-Control Studies
Interleukin-4 Receptor alpha Subunit
/ genetics
Male
Female
Child
Malaria
/ genetics
Child, Preschool
Gene Frequency
Genetic Predisposition to Disease
Genotype
Infant
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide
Promoter Regions, Genetic
Ghana
Interleukin-4
Interleukin-4 receptor
Malaria
Single nucleotide polymorphism
Journal
BMC research notes
ISSN: 1756-0500
Titre abrégé: BMC Res Notes
Pays: England
ID NLM: 101462768
Informations de publication
Date de publication:
31 Oct 2024
31 Oct 2024
Historique:
received:
28
04
2024
accepted:
22
10
2024
medline:
1
11
2024
pubmed:
1
11
2024
entrez:
1
11
2024
Statut:
epublish
Résumé
This study investigated gene polymorphisms in the interleukin-4 (IL-4) and its receptor alpha (IL-4Rα) gene regions in human hosts with uncomplicated malaria. Blood samples were obtained from a case-control study conducted at the Sogakope district hospital in the Volta region of Ghana. Thick blood films were made and used to detect the presence and levels of parasitaemia in the patient samples. Genotyping of IL-4 (150 C/T) and the IL-4Rα (Pro-478-Ser) polymorphisms in the promoter regions and receptor gene was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) after DNA extraction. The data are useful in determining genetic polymorphisms (allele and genotypic frequencies) of IL-4 and its alpha receptor. In addition, they are useful when comparing levels of parasite density and haematological parameters between genotypic variants of IL-4 and IL-4Rα. These data contribute to our understanding of the genetic basis of malaria susceptibility, particularly in the population of the Volta region of Ghana.
Identifiants
pubmed: 39482779
doi: 10.1186/s13104-024-06984-5
pii: 10.1186/s13104-024-06984-5
doi:
Substances chimiques
Interleukin-4
207137-56-2
Interleukin-4 Receptor alpha Subunit
0
IL4 protein, human
0
IL4R protein, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
328Informations de copyright
© 2024. The Author(s).
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