Coiled-coil oligomerization controls localization of the plasma membrane REMORINs.
Amino Acid Sequence
Arabidopsis Proteins
/ chemistry
Cell Membrane
/ metabolism
Cryoelectron Microscopy
Magnetic Resonance Spectroscopy
Microscopy, Confocal
Microscopy, Electron, Transmission
Models, Molecular
Molecular Conformation
Plant Proteins
/ chemistry
Protein Multimerization
Protein Structure, Secondary
Recombinant Proteins
/ chemistry
Sequence Homology, Amino Acid
Membrane protein
Nanodomain
Protein filament
REMORIN
Solid-state NMR
Journal
Journal of structural biology
ISSN: 1095-8657
Titre abrégé: J Struct Biol
Pays: United States
ID NLM: 9011206
Informations de publication
Date de publication:
01 04 2019
01 04 2019
Historique:
received:
19
11
2017
revised:
25
01
2018
accepted:
12
02
2018
pubmed:
27
2
2018
medline:
2
6
2020
entrez:
27
2
2018
Statut:
ppublish
Résumé
REMORINs are nanodomain-organized proteins located in the plasma membrane and involved in cellular responses in plants. The dynamic assembly of the membrane nanodomains represents an essential tool of the versatile membrane barriers to control and modulate cellular functions. Nevertheless, the assembly mechanisms and protein organization strategies of nanodomains are poorly understood and many structural aspects are difficult to visualize. Using an ensemble of biophysical approaches, including solid-state nuclear magnetic resonance, cryo-electron microscopy and in vivo confocal imaging, we provide first insights on the role and the structural mechanisms of REMORIN trimerization. Our results suggest that the formation of REMORIN coiled-coil trimers is essential for membrane recruitment and promotes REMORIN assembly in vitro into long filaments by trimer-trimer interactions that might participate in nanoclustering into membrane domains in vivo.
Identifiants
pubmed: 29481850
pii: S1047-8477(18)30046-7
doi: 10.1016/j.jsb.2018.02.003
pii:
doi:
Substances chimiques
Arabidopsis Proteins
0
Plant Proteins
0
Recombinant Proteins
0
remorin
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
12-19Informations de copyright
Copyright © 2018 Elsevier Inc. All rights reserved.