Resveratrol and ivacaftor are additive G551D CFTR-channel potentiators: therapeutic implications for cystic fibrosis sinus disease.


Journal

International forum of allergy & rhinology
ISSN: 2042-6984
Titre abrégé: Int Forum Allergy Rhinol
Pays: United States
ID NLM: 101550261

Informations de publication

Date de publication:
01 2019
Historique:
received: 03 03 2018
revised: 19 06 2018
accepted: 15 07 2018
pubmed: 29 8 2018
medline: 18 12 2019
entrez: 29 8 2018
Statut: ppublish

Résumé

Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene result in defective Cl Fisher rat thyroid cells (FRT) transfected with G551D CFTR and human sinonasal epithelial cells (HSNE) containing the CFTR G551D mutation were subjected to pharmacologic manipulation of transepithelial ion transport in Ussing chambers. Activity was further evaluated using whole-cell patch clamp methods in G551D FRT cells. In G551D FRT cells, resveratrol (100 μM) and ivacaftor (10 μM) significantly increased Cl Additive improvement in G551D CFTR-mediated Cl

Sections du résumé

BACKGROUND
Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene result in defective Cl
METHODS
Fisher rat thyroid cells (FRT) transfected with G551D CFTR and human sinonasal epithelial cells (HSNE) containing the CFTR G551D mutation were subjected to pharmacologic manipulation of transepithelial ion transport in Ussing chambers. Activity was further evaluated using whole-cell patch clamp methods in G551D FRT cells.
RESULTS
In G551D FRT cells, resveratrol (100 μM) and ivacaftor (10 μM) significantly increased Cl
CONCLUSION
Additive improvement in G551D CFTR-mediated Cl

Identifiants

pubmed: 30152192
doi: 10.1002/alr.22202
pmc: PMC6318032
mid: NIHMS984861
doi:

Substances chimiques

Aminophenols 0
CFTR protein, human 0
Chlorides 0
Quinolones 0
Cystic Fibrosis Transmembrane Conductance Regulator 126880-72-6
ivacaftor 1Y740ILL1Z
Resveratrol Q369O8926L

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

100-105

Subventions

Organisme : NIDDK NIH HHS
ID : P30 DK072482
Pays : United States
Organisme : NHLBI NIH HHS
ID : R35 HL135816
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL133006
Pays : United States

Informations de copyright

© 2018 ARS-AAOA, LLC.

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Auteurs

Do-Yeon Cho (DY)

Department of Otolaryngology, University of Alabama at Birmingham, Birmingham, AL.
Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, AL.

Shaoyan Zhang (S)

Department of Otolaryngology, University of Alabama at Birmingham, Birmingham, AL.
Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, AL.

Ahmed Lazrak (A)

Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, AL.
Department of Anesthesiology and Perioperative Medicine, University of Alabama at Birmingham, Birmingham, AL.

Jessica W Grayson (JW)

Department of Otolaryngology, University of Alabama at Birmingham, Birmingham, AL.

Jaime A Peña Garcia (JA)

Department of Otolaryngology, University of Alabama at Birmingham, Birmingham, AL.

Daniel F Skinner (DF)

Department of Otolaryngology, University of Alabama at Birmingham, Birmingham, AL.
Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, AL.

Dong Jin Lim (DJ)

Department of Otolaryngology, University of Alabama at Birmingham, Birmingham, AL.
Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, AL.

Calvin Mackey (C)

Department of Otolaryngology, University of Alabama at Birmingham, Birmingham, AL.
Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, AL.

Catherine Banks (C)

Department of Otolaryngology, University of Alabama at Birmingham, Birmingham, AL.

Sadis Matalon (S)

Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, AL.
Department of Anesthesiology and Perioperative Medicine, University of Alabama at Birmingham, Birmingham, AL.

Bradford A Woodworth (BA)

Department of Otolaryngology, University of Alabama at Birmingham, Birmingham, AL.
Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, AL.

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