A fully online sensor-equipped, disposable multiphase microbioreactor as a screening platform for biotechnological applications.
biosensor
microbioreactor (MBR)
optical sensor
real-time online analytics
screening platform
sensor integration
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
22
03
2018
revised:
08
08
2018
accepted:
05
09
2018
pubmed:
11
9
2018
medline:
18
12
2019
entrez:
11
9
2018
Statut:
ppublish
Résumé
A new disposable, multiphase, microbioreactor (MBR; with a working volume of 550 μl) equipped with online sensors is presented for biotechnological screening research purposes owing to its high-throughput potential. Its design and fabrication, online sensor integration, and operation are described. During aerobic cultivation, sufficient oxygen supply is the most important factor that influences growth and product formation. The MBR is a microbubble column bioreactor (μBC), and the oxygen supply was realized by active pneumatic bubble aeration, ensuring sufficient volumetric liquid-phase mass transfer (k
Substances chimiques
Biological Products
0
Culture Media
0
Glucose
IY9XDZ35W2
Oxygen
S88TT14065
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
65-75Informations de copyright
© 2018 Wiley Periodicals, Inc.