The green microalga Lobosphaera incisa harbours an arachidonate 15S-lipoxygenase.
Arachidonate 15-Lipoxygenase
/ metabolism
Catalytic Domain
Chloroplasts
/ enzymology
Cloning, Molecular
Fatty Acids, Omega-3
/ metabolism
Hydrogen-Ion Concentration
Kinetics
Microalgae
/ enzymology
Models, Biological
Mutation
/ genetics
Oxidation-Reduction
Oxygen
/ metabolism
Phylogeny
Stereoisomerism
Substrate Specificity
Parietochloris incisa
Fatty acid hydroperoxide
lipid peroxidation
oxylipin formation
positional specificity
substrate orientation
Journal
Plant biology (Stuttgart, Germany)
ISSN: 1438-8677
Titre abrégé: Plant Biol (Stuttg)
Pays: England
ID NLM: 101148926
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
31
07
2018
accepted:
22
09
2018
pubmed:
3
10
2018
medline:
10
1
2019
entrez:
3
10
2018
Statut:
ppublish
Résumé
The green microalga Lobosphaera incisa is an oleaginous eukaryotic alga that is rich in arachidonic acid (20:4). Being rich in this polyunsaturated fatty acid (PUFA), however, makes it sensitive to oxidation. In plants, lipoxygenases (LOXs) are the major enzymes that oxidise these molecules. Here, we describe, to our best knowledge, the first characterisation of a cDNA encoding a LOX (LiLOX) from a green alga. To obtain first insights into its function, we expressed it in E. coli, purified the recombinant enzyme and analysed its enzyme activity. The protein sequence suggests that LiLOX and plastidic LOXs from bryophytes and flowering plants may share a common ancestor. The fact that LiLOX oxidises all PUFAs tested with a consistent oxidation on the carbon n-6, suggests that PUFAs enter the substrate channel through their methyl group first (tail first). Additionally, LiLOX form the fatty acid hydroperoxide in strict S configuration. LiLOX may represent a good model to study plastid LOX, because it is stable after heterologous expression in E. coli and highly active in vitro. Moreover, as the first characterised LOX from green microalgae, it opens the possibility to study endogenous LOX pathways in these organisms.
Identifiants
pubmed: 30277010
doi: 10.1111/plb.12920
pmc: PMC6587457
doi:
Substances chimiques
Fatty Acids, Omega-3
0
Arachidonate 15-Lipoxygenase
EC 1.13.11.33
Oxygen
S88TT14065
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
131-142Subventions
Organisme : German Research Foundation (DFG)
ID : GRK 1422
Organisme : FP7 Food, Agriculture and Fisheries, Biotechnology
ID : 266401
Informations de copyright
© 2018 The Authors. Plant Biology published by John Wiley & Sons Ltd on behalf of German Society for Plant Sciences, Royal Botanical Society of the Netherlands.
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