Functional Reconstitution of Intracellular Vesicle Fusion Using Purified SNAREs and Sec1/Munc18 (SM) Proteins.

Cytoplasmic Vesicles / metabolism Exocytosis Glucose Transporter Type 4 / metabolism Kinetics Membrane Fusion Munc18 Proteins / isolation & purification Recombinant Proteins / isolation & purification SNARE Proteins / isolation & purification
Content mixing Lipid mixing Macromolecular crowding Membrane fusion Reconstitution SM protein SNARE Vesicle fusion

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2019
Historique:
entrez: 15 10 2018
pubmed: 15 10 2018
medline: 7 6 2019
Statut: ppublish

Résumé

The fusion of intracellular vesicles with target membranes is mediated by two classes of conserved molecules-soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAP receptors or SNAREs) and Sec1/Munc18 (SM) proteins. A conserved function of SM proteins is to recognize their cognate trans-SNARE complexes and accelerate fusion kinetics. Here, we describe a physiologically relevant reconstitution system in which macromolecular crowding agents are included to recapitulate the crowded intracellular environment. Through this system, we elucidate the molecular mechanisms by which SNAREs and SM proteins drive vesicle fusion.

Identifiants

DOI: 10.1007/978-1-4939-8760-3_15 PMID: 30317509 PMC: PMC6869331
pubmed: 30317509
doi: 10.1007/978-1-4939-8760-3_15
pmc: PMC6869331
mid: NIHMS1059360
doi:

Substances chimiques

Glucose Transporter Type 4 0
Munc18 Proteins 0
Recombinant Proteins 0
SNARE Proteins 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

237-249

Subventions

Organisme : NIDDK NIH HHS
ID : R01 DK095367
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM126960
Pays : United States
Organisme : NIDDK NIH HHS
ID : R56 DK095367
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM102217
Pays : United States
Organisme : NIA NIH HHS
ID : RF1 AG061829
Pays : United States

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Auteurs

Haijia Yu (H)

Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, China. haijia.yu@colorado.edu.
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO, USA. haijia.yu@colorado.edu.

Lauren Crisman (L)

Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO, USA.

Michael H B Stowell (MHB)

Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO, USA.

Jingshi Shen (J)

Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO, USA. jingshi.shen@colorado.edu.

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Classifications MeSH

questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Vésicules cytoplasmiques › Functional Reconstitution of Intracellular...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Exocytose › Functional Reconstitution of Intracellular...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Protéines transporteurs de solutés › Transporteurs de glucose par diffusion facilitée › Transporteur de glucose de type 4 › Functional Reconstitution of Intracellular...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › Functional Reconstitution of Intracellular...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Fusion membranaire › Functional Reconstitution of Intracellular...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines du transport vésiculaire › Protéines Munc18 › Functional Reconstitution of Intracellular...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Functional Reconstitution of Intracellular...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines du transport vésiculaire › Protéines SNARE › Functional Reconstitution of Intracellular...