The Arabidopsis RRM domain protein EDM3 mediates race-specific disease resistance by controlling H3K9me2-dependent alternative polyadenylation of RPP7 immune receptor transcripts.

Arabidopsis / genetics Arabidopsis Proteins / genetics Gene Expression Regulation, Plant / genetics RNA-Binding Proteins / genetics
Hyaloperonospora arabidopsidis RNA-binding protein disease-resistance genes histone-binding proteins transcript processing

Journal

The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397

Informations de publication

Date de publication:
02 2019
Historique:
received: 06 06 2018
revised: 27 09 2018
accepted: 26 10 2018
pubmed: 9 11 2018
medline: 23 2 2020
entrez: 9 11 2018
Statut: ppublish

Résumé

The NLR-receptor RPP7 mediates race-specific immunity in Arabidopsis. Previous screens for enhanced downy mildew (edm) mutants identified the co-chaperone SGT1b (EDM1) and the PHD-finger protein EDM2 as critical regulators of RPP7. Here, we describe a third edm mutant compromised in RPP7 immunity, edm3. EDM3 encodes a nuclear-localized protein featuring an RNA-recognition motif. Like EDM2, EDM3 promotes histone H3 lysine 9 dimethylation (H3K9me2) at RPP7. Global profiling of H3K9me2 showed EDM3 to affect this silencing mark at a large set of loci. Importantly, both EDM3 and EDM2 co-associate in vivo with H3K9me2-marked chromatin and transcripts at a critical proximal polyadenylation site of RPP7, where they suppress proximal transcript polyadeylation/termination. Our results highlight the complexity of plant NLR gene regulation, and establish a functional and physical link between a histone mark and NLR-transcript processing.

Identifiants

DOI: 10.1111/tpj.14148 PMID: 30407670 PMC: PMC7138032
pubmed: 30407670
doi: 10.1111/tpj.14148
pmc: PMC7138032
mid: NIHMS1017375
doi:

Substances chimiques

Arabidopsis Proteins 0
RNA-Binding Proteins 0
RPP7 protein, Arabidopsis 0

Banques de données

GENBANK
['GSE108490', 'SRR5515902', 'SRR5515903', 'SRR5515906', 'SRR5515907']

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

646-660

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI106775
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI136511
Pays : United States
Organisme : Biotechnology and Biological Science Research Council
ID : 207/ICR07554
Pays : International

Informations de copyright

© 2018 The Authors The Plant Journal © 2018 John Wiley & Sons Ltd.

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Auteurs

Yan Lai (Y)

Department of Botany and Plant Sciences, Center for Plant Cell Biology, Institute of Integrative Genome Biology, University of California at Riverside, Riverside, CA, 92521, USA.
College of Life Sciences, Fujian Agricultural and Forestry University, Fuzhou, Fujian, 350002, China.

Alayne Cuzick (A)

School of Life Sciences, University of Warwick, Wellesbourne Campus, Warwick, CV35 9EF, UK.
Biointeractions and Crop Protection, Rothamsted Research, Harpenden, AL5 2JQ, UK.

Xueqing M Lu (XM)

Department of Molecular, Cell and Systems Biology, Center for Infectious Disease and Vector Research, Institute of Integrative Genome Biology, University of California at Riverside, Riverside, CA, 92521, USA.

Jianqiang Wang (J)

Department of Botany and Plant Sciences, Center for Plant Cell Biology, Institute of Integrative Genome Biology, University of California at Riverside, Riverside, CA, 92521, USA.

Neerja Katiyar (N)

Institute of Integrative Genome Biology, University of California at Riverside, Riverside, CA, 92521, USA.

Tokuji Tsuchiya (T)

College of Bioresource Sciences, Nihon University, Kanagawa, 252-0880, Japan.

Karine Le Roch (K)

Department of Molecular, Cell and Systems Biology, Center for Infectious Disease and Vector Research, Institute of Integrative Genome Biology, University of California at Riverside, Riverside, CA, 92521, USA.

John M McDowell (JM)

Department of Plant Pathology, Physiology, and Weed Science, Virginia Tech, Blacksburg, VA, 24060-0329, USA.

Eric Holub (E)

School of Life Sciences, University of Warwick, Wellesbourne Campus, Warwick, CV35 9EF, UK.

Thomas Eulgem (T)

Department of Botany and Plant Sciences, Center for Plant Cell Biology, Institute of Integrative Genome Biology, University of California at Riverside, Riverside, CA, 92521, USA.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Brassicaceae › Arabidopsis › The Arabidopsis RRM domain protein EDM3...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Protéines d'Arabidopsis › The Arabidopsis RRM domain protein EDM3...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes végétaux › The Arabidopsis RRM domain protein EDM3...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Protéines de liaison à l'ARN › The Arabidopsis RRM domain protein EDM3...