Characterization and functional analysis of grouper (Epinephelus coioides) MEK1 and MEK2.

Amino Acid Sequence Animals Bass / genetics Fish Diseases / immunology Fish Proteins / chemistry Gene Expression Profiling / veterinary Gene Expression Regulation / immunology Immunity, Innate / genetics MAP Kinase Kinase 1 / genetics MAP Kinase Kinase 2 / genetics Phylogeny Sequence Alignment / veterinary
AP1 Cryptocaryon irritans ERK1/2 Epinephelus coioides MEK1/2

Journal

Fish & shellfish immunology
ISSN: 1095-9947
Titre abrégé: Fish Shellfish Immunol
Pays: England
ID NLM: 9505220

Informations de publication

Date de publication:
Jan 2019
Historique:
received: 15 08 2018
revised: 02 11 2018
accepted: 05 11 2018
pubmed: 13 11 2018
medline: 16 4 2019
entrez: 13 11 2018
Statut: ppublish

Résumé

MEK dual-specificity protein kinases are a group of mitogen-activated protein kinase kinases, which act as an integration point by transferring extracellular signals to the nucleus. To investigate the function of MEK in teleost fish, we cloned MEK1 and MEK2 cDNA sequences from the orange-spotted grouper (Epinephelus coioides). EcMEK1 and EcMEK2 shared 80% amino acid identity with each other. EcMEK1 had 89-99% amino acid identity with teleosts or mammals, whereas EcMEK2 shared 85-97% amino acid identity. The exon structures of the grouper MEK1/2 genes were conserved with zebrafish and human MEK1/2. Tissue distribution analysis showed that EcMEK1 and EcMEK2 had a similar expression pattern in grouper tissues and was mainly transcribe in systemic immune organs. Both EcMEK1 and EcMEK2 were distributed throughout the cytoplasm of transfected GS or HEK293T cells. Overexpression of EcMEK1 or EcMEK2 activated Activator protein 1 dependent luciferase. The phosphorylation levels of EcMEK1/2 and EcERK1/2 were significantly increased in head kidney leukocytes by stimulation with PMA treatment. The grouper MEK1/2-ERK1/2 axis was activated in Cryptocaryon irritans infection and showed an enhanced phosphorylation after immunization.

Identifiants

DOI: 10.1016/j.fsi.2018.11.016 PMID: 30419398
pubmed: 30419398
pii: S1050-4648(18)30735-6
doi: 10.1016/j.fsi.2018.11.016
pii:
doi:

Substances chimiques

Fish Proteins 0
MAP Kinase Kinase 1 EC 2.7.12.2
MAP Kinase Kinase 2 EC 2.7.12.2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1090-1097

Informations de copyright

Copyright © 2018 Elsevier Ltd. All rights reserved.

Auteurs

Ze-Quan Mo (ZQ)

Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, Guangdong Province, PR China.

Rui Han (R)

Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, Guangdong Province, PR China.

Jiu-Le Wang (JL)

Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, Guangdong Province, PR China.

Lu-Yun Ni (LY)

Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, Guangdong Province, PR China.

Yu-Ling Su (YL)

Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, Guangdong Province, PR China.

Xue-Li Lai (XL)

Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, Guangdong Province, PR China.

Zhi-Chang He (ZC)

Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, Guangdong Province, PR China.

Hong-Ping Chen (HP)

Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, Guangdong Province, PR China.

Yan-Wei Li (YW)

Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, Guangdong Province, PR China.

Hong-Yan Sun (HY)

Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, Guangdong Province, PR China.

Xiao-Chun Luo (XC)

School of Bioscience and Biotechnology, South China University of Technology, Guangzhou, 510006, PR China. Electronic address: xcluo@scut.edu.cn.

Xue-Ming Dan (XM)

Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, Guangdong Province, PR China. Electronic address: dxm72@scau.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › Characterization and functional analysis of...
questionsmedicales.fr › Eucaryotes › Animaux › Characterization and functional analysis of...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Poissons › Perciformes › Serran › Characterization and functional analysis of...
questionsmedicales.fr › Maladies de l'animal › Maladies des poissons › Characterization and functional analysis of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de poisson › Characterization and functional analysis of...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de profil d'expression de gènes › Characterization and functional analysis of...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Characterization and functional analysis of...
questionsmedicales.fr › Phénomènes du système immunitaire › Immunité › Immunité innée › Characterization and functional analysis of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protein-tyrosine kinases › Mitogen-Activated Protein Kinase Kinases › MAP Kinase Kinase 1 › Characterization and functional analysis of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protein-tyrosine kinases › Mitogen-Activated Protein Kinase Kinases › MAP Kinase Kinase 2 › Characterization and functional analysis of...
questionsmedicales.fr › Sciences de l'information › Phylogenèse › Characterization and functional analysis of...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Alignement de séquences › Characterization and functional analysis of...