Propeptide glycosylation and galectin-3 binding decrease proteolytic activation of human proMMP-9/progelatinase B.


Journal

The FEBS journal
ISSN: 1742-4658
Titre abrégé: FEBS J
Pays: England
ID NLM: 101229646

Informations de publication

Date de publication:
03 2019
Historique:
received: 05 07 2018
revised: 21 09 2018
accepted: 09 11 2018
pubmed: 14 11 2018
medline: 18 12 2019
entrez: 14 11 2018
Statut: ppublish

Résumé

Matrix metalloproteinases (MMPs) are secreted as proenzymes, containing propeptides that interact with the catalytic zinc, thereby controlling MMP activation. The MMP-9 propeptide is unique in the MMP family because of its post-translational modification with an N-linked oligosaccharide. ProMMP-9 activation by MMP-3 occurs stepwise by cleavage of the propeptide in an aminoterminal (pro-AT) and carboxyterminal (pro-CT) peptide. We chemically synthesized aglycosyl pro-AT and pro-CT and purified recombinant glycosylated pro-AT

Identifiants

pubmed: 30422384
doi: 10.1111/febs.14698
pmc: PMC7379967
doi:

Substances chimiques

Blood Proteins 0
Enzyme Precursors 0
Galectin 3 0
Galectins 0
LGALS3 protein, human 0
Leukocyte Elastase EC 3.4.21.37
Gelatinases EC 3.4.24.-
progelatinase EC 3.4.24.-
MMP3 protein, human EC 3.4.24.17
Matrix Metalloproteinase 3 EC 3.4.24.17
MMP9 protein, human EC 3.4.24.35
Matrix Metalloproteinase 9 EC 3.4.24.35

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

930-945

Informations de copyright

© 2018 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.

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Auteurs

Lise Boon (L)

Laboratory of Immunobiology, Rega Institute for Medical Research, KU Leuven, Belgium.

Estefania Ugarte-Berzal (E)

Laboratory of Immunobiology, Rega Institute for Medical Research, KU Leuven, Belgium.

Erik Martens (E)

Laboratory of Immunobiology, Rega Institute for Medical Research, KU Leuven, Belgium.

Jennifer Vandooren (J)

Laboratory of Immunobiology, Rega Institute for Medical Research, KU Leuven, Belgium.

Vasily Rybakin (V)

Laboratory of Immunobiology, Rega Institute for Medical Research, KU Leuven, Belgium.

Didier Colau (D)

Ludwig Institute for Cancer Research, Brussels, Belgium.

Monica Gordon-Alonso (M)

Institut de Duve, UCLouvain, Brussels, Belgium.

Pierre van der Bruggen (P)

Institut de Duve, UCLouvain, Brussels, Belgium.

Walter Stöcker (W)

Institute of Molecular Physiology, Johannes Gutenberg University, Mainz, Germany.

Christoph Becker-Pauly (C)

Biochemical Institute, University of Kiel, Germany.

Peter Witters (P)

Department of Pediatrics, University Hospitals Leuven, KU Leuven, Belgium.
Department of Development and Regeneration, KU Leuven, Belgium.

Eva Morava (E)

Department of Clinical Genomics, Mayo Clinic, Rochester, ON, USA.

Jaak Jaeken (J)

Department of Pediatrics, University Hospitals Leuven, KU Leuven, Belgium.
Department of Development and Regeneration, KU Leuven, Belgium.

Paul Proost (P)

Laboratory of Molecular Immunology, Rega Institute for Medical Research, KU Leuven, Belgium.

Ghislain Opdenakker (G)

Laboratory of Immunobiology, Rega Institute for Medical Research, KU Leuven, Belgium.

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Classifications MeSH