Impedimetric mechanism study of horseradish peroxidase at low and high concentrations of hydrogen peroxide based on graphene/sol-gel/horseradish peroxidase.
Hydrogen peroxidas
Hydrogen peroxide
Impedimetric
Mechanism
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
15 Feb 2019
15 Feb 2019
Historique:
received:
09
10
2018
revised:
09
11
2018
accepted:
11
11
2018
pubmed:
16
11
2018
medline:
19
4
2019
entrez:
16
11
2018
Statut:
ppublish
Résumé
A novel electrochemical modified electrode for the impedimetric mechanism study of horseradish peroxidase and hydrogen peroxide was proposed based on immobilizing horseradish peroxidase/3‑(trimethoxysilyl) propylamine/graphene on glassy carbon electrode (HRP/TMSPA/Gr/GCE). Cyclic voltammetry and SEM were utilized to corroborate the successful stepwise assemblage procedure of the electrode. The electrochemical impedance spectroscopy measurements revealed that the charge transfer resistance increases at low concentrations and significantly decreases at high concentrations after enzymatic reaction with hydrogen peroxide. The mechanism study of the horseradish peroxidase and hydrogen peroxide were also investigated by electrochemical impedance spectroscopy.
Identifiants
pubmed: 30439427
pii: S0141-8130(18)35407-2
doi: 10.1016/j.ijbiomac.2018.11.091
pii:
doi:
Substances chimiques
Enzymes, Immobilized
0
Nanotubes, Carbon
0
Graphite
7782-42-5
Hydrogen Peroxide
BBX060AN9V
Horseradish Peroxidase
EC 1.11.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
677-681Informations de copyright
Copyright © 2018 Elsevier B.V. All rights reserved.