Characterization of N-glycosylations in Entamoeba histolytica ubiquitin.

Electrophoresis, Polyacrylamide Gel Entamoeba histolytica / chemistry Gas Chromatography-Mass Spectrometry Glycosylation Mass Spectrometry / methods Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / metabolism Periodic Acid-Schiff Reaction Polysaccharides / chemistry Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Trophozoites / chemistry Ubiquitin / genetics
Continuous elution electrophoresis Entamoeba histolytica GC-MS MALDI-TOF MS N-Glycans NSI-MS/MS Ubiquitin

Journal

Experimental parasitology
ISSN: 1090-2449
Titre abrégé: Exp Parasitol
Pays: United States
ID NLM: 0370713

Informations de publication

Date de publication:
Jan 2019
Historique:
received: 14 04 2018
revised: 10 11 2018
accepted: 13 11 2018
pubmed: 18 11 2018
medline: 16 1 2019
entrez: 17 11 2018
Statut: ppublish

Résumé

Entamoeba histolytica harbors an extensive intracellular distribution of ubiquitin-proteasome systems important for numerous cellular processes. However, glycosylation studies of ubiquitin-proteasome components have not yet been elucidated. Here we report the partial characterization of N-linked glycosylation profile in E. histolytica ubiquitin by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), Nanoelectrospray Ionization-Tandem Mass Spectrometry (NSI-MS), Matrix-Assisted Laser-Desorption time-of-flight Mass Spectrometry (MALDI-TOF MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis. To our knowledge, the data presented in this report represents the first structural glycomics analysis of E. histolytica ubiquitin, while most of the reports are performed on whole parasitic glycan profiles. The glycan profile of E. histolytica ubiquitin has high mannose N-glycan structures. The N-linked glycan profile showed fragments from Hex

Identifiants

DOI: 10.1016/j.exppara.2018.11.003 PMID: 30444974
pubmed: 30444974
pii: S0014-4894(18)30193-0
doi: 10.1016/j.exppara.2018.11.003
pii:
doi:

Substances chimiques

Polysaccharides 0
Ubiquitin 0
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase EC 3.5.1.52

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

38-47

Informations de copyright

Copyright © 2018 Elsevier Inc. All rights reserved.

Auteurs

Adriana Obregón (A)

Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Instituto de Biotecnología, San Nicolás de los Garza, Nuevo León, Mexico. Electronic address: adriana_obr@hotmail.com.

María S Flores (MS)

Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Instituto de Biotecnología, San Nicolás de los Garza, Nuevo León, Mexico. Electronic address: maria.floresgz@uanl.edu.mx.

Roberto Rangel (R)

Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Instituto de Biotecnología, San Nicolás de los Garza, Nuevo León, Mexico. Electronic address: rrangel@mdanderson.org.

Katiuska Arévalo (K)

Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Instituto de Biotecnología, San Nicolás de los Garza, Nuevo León, Mexico. Electronic address: karevalo01@hotmail.com.

Guadalupe Maldonado (G)

Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Instituto de Biotecnología, San Nicolás de los Garza, Nuevo León, Mexico. Electronic address: mgpemald@hotmail.com.

Isela Quintero (I)

Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Instituto de Biotecnología, San Nicolás de los Garza, Nuevo León, Mexico. Electronic address: qisela@hotmail.com.

Luis Galán (L)

Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Instituto de Biotecnología, San Nicolás de los Garza, Nuevo León, Mexico. Electronic address: luis.galanwn@uanl.edu.mx.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques électrochimiques › Électrophorèse › Électrophorèse sur gel de polyacrylamide › Characterization of N-glycosylations in...
questionsmedicales.fr › Eucaryotes › Amoebozoa › Lobosea › Amoebida › Tubulina › Entamoeba › Entamoeba histolytica › Characterization of N-glycosylations in...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Chromatographie gazeuse-spectrométrie de masse › Characterization of N-glycosylations in...
questionsmedicales.fr › Métabolisme › Métabolisme glucidique › Glycosylation › Characterization of N-glycosylations in...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Characterization of N-glycosylations in...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Amidohydrolases › Peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase › Characterization of N-glycosylations in...
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques histologiques › Réaction à l'acide periodique de Schiff › Characterization of N-glycosylations in...
questionsmedicales.fr › Glucides › Polyosides › Characterization of N-glycosylations in...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse MALDI › Characterization of N-glycosylations in...
questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › Morphogenèse › Métamorphose biologique › Étapes du cycle de vie › Trophozoïtes › Characterization of N-glycosylations in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Ubiquitines › Ubiquitine › Characterization of N-glycosylations in...