Forensic tri-allelic SNP genotyping using nanopore sequencing.

Alleles DNA Primers Genotyping Techniques / methods High-Throughput Nucleotide Sequencing Humans Multiplex Polymerase Chain Reaction Nanopores Polymorphism, Single Nucleotide Sequence Analysis, DNA
Forensic MinION Next generation sequencing Oxford nanopore technologies SNP

Journal

Forensic science international. Genetics
ISSN: 1878-0326
Titre abrégé: Forensic Sci Int Genet
Pays: Netherlands
ID NLM: 101317016

Informations de publication

Date de publication:
01 2019
Historique:
received: 03 04 2018
revised: 09 11 2018
accepted: 09 11 2018
pubmed: 19 11 2018
medline: 27 1 2019
entrez: 19 11 2018
Statut: ppublish

Résumé

The potential and current state-of-the-art of forensic SNP genotyping using nanopore sequencing was investigated with a panel of 16 tri-allelic single nucleotide polymorphisms (SNPs), multiplexing five samples per sequencing run. The sample set consisted of three single-source human genomic reference control DNA samples and two GEDNAP samples, simulating casework samples. The primers for the multiplex SNP-loci PCR were taken from a study which researched their value in a forensic setting using conventional single-base extension technology. Workflows for multiplexed Oxford Nanopore Technologies' 1D and 1D

Identifiants

DOI: 10.1016/j.fsigen.2018.11.012 PMID: 30448528
pubmed: 30448528
pii: S1872-4973(18)30150-9
doi: 10.1016/j.fsigen.2018.11.012
pii:
doi:

Substances chimiques

DNA Primers 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

204-210

Informations de copyright

Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Auteurs

Senne Cornelis (S)

Laboratory of Pharmaceutical Biotechnology, Ghent University, 9000 Gent, Belgium; Department of Life Sciences and Imaging, imec, 3001 Leuven, Belgium. Electronic address: Senne.Cornelis@UGent.be.

Yannick Gansemans (Y)

Laboratory of Pharmaceutical Biotechnology, Ghent University, 9000 Gent, Belgium. Electronic address: Yannick.Gansemans@UGent.be.

Ann-Sophie Vander Plaetsen (AS)

Laboratory of Pharmaceutical Biotechnology, Ghent University, 9000 Gent, Belgium. Electronic address: AnnSophie.VanderPlaetsen@UGent.be.

Jana Weymaere (J)

Laboratory of Pharmaceutical Biotechnology, Ghent University, 9000 Gent, Belgium. Electronic address: Jana.Weymaere@UGent.be.

Sander Willems (S)

Laboratory of Pharmaceutical Biotechnology, Ghent University, 9000 Gent, Belgium. Electronic address: Sander.Willems@UGent.be.

Dieter Deforce (D)

Laboratory of Pharmaceutical Biotechnology, Ghent University, 9000 Gent, Belgium. Electronic address: Dieter.Deforce@UGent.be.

Filip Van Nieuwerburgh (F)

Laboratory of Pharmaceutical Biotechnology, Ghent University, 9000 Gent, Belgium. Electronic address: Filip.VanNieuwerburgh@UGent.be.

Articles similaires

Comprehensive comparative analysis and development of molecular markers for Lasianthus species based on complete chloroplast genome sequences.

Yue Zhang, Meifang Song, Deying Tang et al.
1.00
Genome, Chloroplast Phylogeny Genetic Markers Base Composition High-Throughput Nucleotide Sequencing

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Allèles › Forensic tri-allelic SNP genotyping using...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Sondes moléculaires › Sondes d'acide nucléique › Sondes d'ADN › Amorces ADN › Forensic tri-allelic SNP genotyping using...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques de génotypage › Forensic tri-allelic SNP genotyping using...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Séquençage nucléotidique à haut débit › Forensic tri-allelic SNP genotyping using...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Forensic tri-allelic SNP genotyping using...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine multiplex › Forensic tri-allelic SNP genotyping using...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Nanostructures › Nanopores › Forensic tri-allelic SNP genotyping using...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Polymorphisme génétique › Polymorphisme de nucléotide simple › Forensic tri-allelic SNP genotyping using...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ADN › Forensic tri-allelic SNP genotyping using...