Microarrays for High-Throughput Gene Expression Analysis of Barley.

Data Analysis Fluorescent Dyes / metabolism Gene Expression Regulation, Plant Hordeum / genetics Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis / methods Quality Control RNA, Plant / isolation & purification Staining and Labeling
Agilent Barley Gene expression Microarrays RNA Transcriptomics

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2019
Historique:
entrez: 22 11 2018
pubmed: 22 11 2018
medline: 8 6 2019
Statut: ppublish

Résumé

Microarrays remain an extremely robust and cost-efficient technology for transcriptome analysis. A custom Agilent microarray has been designed from the predicted gene transcripts of the barley genome reference. Here, we describe total RNA extraction, fluorescent labeling, hybridization, and image analysis of barley arrays. Quality control measures are detailed, along with recommendations for downstream data analysis.

Identifiants

DOI: 10.1007/978-1-4939-8944-7_12 PMID: 30460566
pubmed: 30460566
doi: 10.1007/978-1-4939-8944-7_12
doi:

Substances chimiques

Fluorescent Dyes 0
RNA, Plant 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

181-194

Auteurs

Jenny A Morris (JA)

Genome Technology, Cell and Molecular Sciences, James Hutton Institute, Dundee, UK.

Pete E Hedley (PE)

Genome Technology, Cell and Molecular Sciences, James Hutton Institute, Dundee, UK. pete.hedley@hutton.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Disciplines des sciences naturelles › Mathématiques › Analyse de données › Microarrays for High-Throughput Gene...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Luminescents › Colorants fluorescents › Microarrays for High-Throughput Gene...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes végétaux › Microarrays for High-Throughput Gene...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Poaceae › Hordeum › Microarrays for High-Throughput Gene...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Hybridation d'acides nucléiques › Microarrays for High-Throughput Gene...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Séquençage par oligonucléotides en batterie › Microarrays for High-Throughput Gene...
questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Contrôle de qualité › Microarrays for High-Throughput Gene...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN des plantes › Microarrays for High-Throughput Gene...
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques histologiques › Techniques de préparation histocytologique › Coloration et marquage › Microarrays for High-Throughput Gene...