Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance.
Antigens, CD34
/ metabolism
Cell Differentiation
/ immunology
Cell Separation
/ instrumentation
Cells, Cultured
Cytokines
/ immunology
Fetal Blood
/ cytology
Flow Cytometry
/ instrumentation
Fluorescent Dyes
/ chemistry
Humans
Immunologic Surveillance
Killer Cells, Natural
/ immunology
Lymphocyte Subsets
/ immunology
Neoplasms
/ immunology
Precursor Cells, T-Lymphoid
/ physiology
Primary Cell Culture
/ instrumentation
Cell isolation
Chemotaxis
Cytofluorimetric analysis
Cytokines
Cytotoxicity
Differentiation
Maturation
Migration
Natural Killer cells
Precursors
Tissues
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
23
11
2018
pubmed:
23
11
2018
medline:
7
6
2019
Statut:
ppublish
Résumé
This chapter will describe the current methodologies to isolate and expand NK cells from Peripheral Blood (PB) or tissues for "in vitro" studies, including NK cell antitumor immune function. In addition, methods to induce NK cell maturation, differentiation, and expansion from CD34
Identifiants
pubmed: 30465197
doi: 10.1007/978-1-4939-8885-3_6
doi:
Substances chimiques
Antigens, CD34
0
Cytokines
0
Fluorescent Dyes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM