Predicting Genes in Single Genomes with AUGUSTUS.
AUGUSTUS
RNA-Seq
evidence integration
gene prediction
genome annotation
Journal
Current protocols in bioinformatics
ISSN: 1934-340X
Titre abrégé: Curr Protoc Bioinformatics
Pays: United States
ID NLM: 101157830
Informations de publication
Date de publication:
03 2019
03 2019
Historique:
pubmed:
23
11
2018
medline:
15
6
2019
entrez:
23
11
2018
Statut:
ppublish
Résumé
AUGUSTUS is a tool for finding protein-coding genes and their exon-intron structure in genomic sequences. It does not necessarily require additional experimental input, as it can be applied in so-called ab initio mode. However, extrinsic evidence from various sources such as transcriptome sequencing or the annotations of closely related genomes can be integrated in order to improve the accuracy and completeness of the annotation. AUGUSTUS can be applied to single genomes, or simultaneously to several aligned genomes. Here, we describe steps required for training AUGUSTUS for the annotation of individual genomes and the steps to do the actual structural annotation. Further, we describe the generation and integration of evidence from various sources of extrinsic evidence. © 2018 by John Wiley & Sons, Inc.
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e57Informations de copyright
© 2018 John Wiley & Sons, Inc.