MicroRNA156 amplifies transcription factor-associated cold stress tolerance in plant cells.
Arabidopsis
/ genetics
Cold Temperature
Cold-Shock Response
/ genetics
Gene Expression Regulation, Plant
/ genetics
Gene Knockdown Techniques
MicroRNAs
/ genetics
Oryza
/ genetics
Plant Cells
/ metabolism
Plant Proteins
/ genetics
Plants, Genetically Modified
/ genetics
Transcription Factors
/ genetics
Cold stress
Luciferase
MiR156
Promoter-binding-like (SPL) genes
WRKY transcription factor
Journal
Molecular genetics and genomics : MGG
ISSN: 1617-4623
Titre abrégé: Mol Genet Genomics
Pays: Germany
ID NLM: 101093320
Informations de publication
Date de publication:
Apr 2019
Apr 2019
Historique:
received:
29
05
2018
accepted:
13
11
2018
pubmed:
28
11
2018
medline:
27
3
2019
entrez:
28
11
2018
Statut:
ppublish
Résumé
MicroRNAs may increase cold stress tolerance by regulating stress-related signal transduction pathways and by modulating the expression of transcription factors. However, the molecular mechanism by which microRNAs enhance cold stress tolerance is not fully understood. Here, we report that overexpression of rice microRNA156 (OsmiR156) results in increased cell viability and growth rate under cold stress in Arabidopsis, pine, and rice. OsmiR156 increases cold stress tolerance by targeting OsSPL3. OsSPL3 positively regulates the expression of OsWRKY71, a negative regulator of the transcription factors OsMYB2 and OsMYB3R-2. OsMYB2 counteracts cold stress by activating the expression of the stress-response genes OsLEA3, OsRab16A, and OsDREB2A. OsMYB3R-2 counteracts cold stress by activating the expression of OsKNOLLE2, OsCTP1, OsCycB1.1, OsCycB2.1, and OsCDC20.1. In OsmiR156 transgenic rice cell lines, the transcript levels of OsLEA3, OsRab16A, OsDREB2A, OsKNOLLE2, OsCTP1, OsCycB1.1, OsCycB2.1, and OsCDC20.1 were increased by OsWRKY71 knockdown and inversely regulated by OsWRKY71 overexpression, indicating that OsmiR156 enhances cold stress tolerance by regulating the expression of transcription factor genes in plant cells. These results will increase our understanding of microRNA-related cold stress tolerance in different plant species, including monocotyledonous, dicotyledonous, and gymnosperm plant species, and will be valuable in plant molecular biotechnology.
Identifiants
pubmed: 30478522
doi: 10.1007/s00438-018-1516-4
pii: 10.1007/s00438-018-1516-4
doi:
Substances chimiques
MicroRNAs
0
Mirn156 microRNA, Arabidopsis
0
Plant Proteins
0
Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
379-393Références
Science. 2002 Apr 5;296(5565):79-92
pubmed: 11935017
Science. 2002 Apr 5;296(5565):92-100
pubmed: 11935018
Cell. 2004 Jan 23;116(2):281-97
pubmed: 14744438
Mol Cell. 2004 Jun 18;14(6):787-99
pubmed: 15200956
Plant Cell. 2004 Aug;16(8):2001-19
pubmed: 15258262
Plant Physiol. 2006 Sep;142(1):280-93
pubmed: 16861571
J Biol Chem. 2006 Dec 8;281(49):37636-45
pubmed: 17015446
J Exp Bot. 2007;58(3):545-54
pubmed: 17158108
BMC Plant Biol. 2008 Jan 29;8:13
pubmed: 18230142
Plant Physiol. 2009 Apr;149(4):1761-72
pubmed: 19211694
Plant Physiol. 2009 May;150(1):244-56
pubmed: 19279197
PLoS Biol. 2009 Jul;7(7):e1000148
pubmed: 19582143
Nature. 2009 Sep 24;461(7263):546-9
pubmed: 19734881
Phytochemistry. 2010 Apr;71(5-6):605-13
pubmed: 20096428
Plant Cell Physiol. 2010 Jun;51(6):1079-83
pubmed: 20460498
Nat Genet. 2010 Jun;42(6):541-4
pubmed: 20495565
Mol Genet Genomics. 2010 Dec;284(6):477-88
pubmed: 20941508
J Exp Bot. 2011 May;62(8):2465-83
pubmed: 21278228
Mol Biotechnol. 2011 Oct;49(2):159-65
pubmed: 21359858
Physiol Plant. 2011 Sep;143(1):1-9
pubmed: 21496029
PLoS One. 2012;7(1):e30039
pubmed: 22253868
J Exp Bot. 2012 Apr;63(7):2541-56
pubmed: 22301384
BMC Plant Biol. 2012 Feb 13;12:18
pubmed: 22330773
Mol Biol Rep. 2012 Sep;39(9):8645-54
pubmed: 22718503
BMC Genomics. 2012 Jul 02;13:290
pubmed: 22747909
Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):18198-203
pubmed: 23071326
Mol Biol Rep. 2013 Mar;40(3):2723-32
pubmed: 23275191
Plant Physiol. 2013 Mar;161(3):1375-91
pubmed: 23292790
Mol Genet Genomics. 2013 Apr;288(3-4):141-55
pubmed: 23539153
PLoS One. 2013;8(3):e59423
pubmed: 23544066
Science. 2013 May 31;340(6136):1094-7
pubmed: 23723236
Gene. 2013 Nov 1;530(1):26-32
pubmed: 23948080
Funct Integr Genomics. 2014 Mar;14(1):75-83
pubmed: 24132512
PLoS One. 2014 Sep 15;9(9):e107678
pubmed: 25222737
PLoS One. 2014 Oct 16;9(10):e109835
pubmed: 25330175
J Exp Bot. 2015 Feb;66(3):789-804
pubmed: 25371507
Plant Sci. 2015 Apr;233:11-21
pubmed: 25711809
Front Plant Sci. 2015 Apr 02;6:208
pubmed: 25883599
Front Plant Sci. 2015 May 13;6:333
pubmed: 26029232
Mol Plant Pathol. 2016 Apr;17(3):427-37
pubmed: 26136043
Plant Mol Biol. 2015 Sep;89(1-2):1-19
pubmed: 26253592
Plant Cell Environ. 2016 Mar;39(3):645-51
pubmed: 26476017
Front Plant Sci. 2015 Nov 05;6:946
pubmed: 26594218
Plant Cell Physiol. 2016 Jan;57(1):192-203
pubmed: 26647245
Plant Physiol. 2016 Feb;170(2):1014-29
pubmed: 26662602
Mol Cells. 2016 Feb;39(2):111-8
pubmed: 26674968
Biochem Biophys Res Commun. 2016 Jan 15;469(3):686-91
pubmed: 26692488
Sci Rep. 2016 Jan 27;6:19736
pubmed: 26813144
PLoS One. 2016 Mar 31;11(3):e0152294
pubmed: 27032112
Plant Physiol Biochem. 2016 Jul;104:200-15
pubmed: 27038155
Front Plant Sci. 2016 May 13;7:658
pubmed: 27242859
Front Plant Sci. 2016 Aug 15;7:1231
pubmed: 27574526
Plant Mol Biol. 2016 Dec;92(6):661-674
pubmed: 27605094
Mol Plant. 2016 Dec 5;9(12):1647-1659
pubmed: 27815142
J Plant Physiol. 2017 Jan;208:52-60
pubmed: 27889521
PLoS One. 2016 Dec 13;11(12):e0167941
pubmed: 27959937
PLoS One. 2017 Jan 26;12(1):e0170578
pubmed: 28125637
BMC Genomics. 2017 Feb 28;18(1):212
pubmed: 28241738
Plant Cell. 2017 Apr;29(4):697-707
pubmed: 28298520
Plant Sci. 2017 May;258:122-136
pubmed: 28330556
Front Plant Sci. 2017 Mar 14;8:356
pubmed: 28352280
Sci Rep. 2017 Apr 06;7:45490
pubmed: 28382938
Plant Biotechnol J. 2018 Jan;16(1):39-49
pubmed: 28436149
Plant Sci. 2017 Jun;259:35-47
pubmed: 28483052
Plant Mol Biol. 1995 Nov;29(4):691-702
pubmed: 8541496